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首页> 外文期刊>Advance journal of food science and technology >Effect of Different Carbon Source on Expression of Carotenogenic Genes and Astaxanthin Production in Xanthophyllomyces dendrorhous
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Effect of Different Carbon Source on Expression of Carotenogenic Genes and Astaxanthin Production in Xanthophyllomyces dendrorhous

机译：不同碳源对黄单胞菌类胡萝卜素基因表达和虾青素产生的影响

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摘要

The present research gives an insight into astaxanthin production, as well as transcription differences of four key carotenogenic genes, in Xanthophyllomyces dendrorhous when cultured with various carbon sources and soybean oil as co-substrates. Glucose was found to be the carbon source with best culture growth and astaxanthin production and the addition of 2% (v/v) soybean oil resulted in even higher astaxanthin producing. In addition, four carotenogenic genes encoding geranylgeranyl diphosphate synthase (crtE), phytoene desaturase (crtI), phytoene synthase lycopene cyclase (crtYB) and astaxanthin synthetase (ast), respectively, were demonstrated to be associated with different transcription levels under various substrates. The present study suggests the effectiveness of manipulating the metabolic regulation by using different carbon sources, in order to improve the production of astaxanthin.
机译：本研究提供了一种深入了解虾青素产生的方法，以及在以各种碳源和大豆油为共底物培养的树黄腐菌中四个主要类胡萝卜素基因的转录差异。发现葡萄糖是培养物生长和虾青素产量最佳的碳源，添加2％（v / v）大豆油可提高虾青素的产量。此外，已证明分别编码香叶基香叶基二磷酸合酶（crtE），并六苯并四氢番茄红素合酶（crtYB）和虾青素合酶（ast）的四个类胡萝卜素基因与不同底物下的不同转录水平相关。本研究表明通过使用不同的碳源来操纵代谢调节的有效性，以提高虾青素的产生。

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