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Physico Chemical Properties and Antioxidant Activity of Roselle Seed Extracts

机译:玫瑰茄种子提取物的理化性质和抗氧化活性

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摘要

The aim of this research was to extract phenolic compounds from defatted Roselle (Hibiscus sabdariffa L.) seed and assess their antioxidant potential. Water, ethanol (30%), methanol (30%) and acetone (30%) were used as solvent for extraction. The proximate composition, total phenolic content and extraction yield were analyzed. Antioxidant efficacies of Roselle seed extract were tested by using 1, 1-Diphenyl-2-Picrylhydrazyl (DPPH), hydroxyl, 2, 2'-azinobis-3-ethylbenzothiaz oline-6-sulfonic acid (ABTS) radicals scavenging capacities and reducing power analysis. Roselle seeds were found to be rich in protein (27.745%), carbohydrates (40.45%) and oil (20.83%). The total phenolic content ranged from 1.66±0.03 to 1.99±0.01 (GAE) mg/g using water and 30% acetone respectively. The highest inhibitory capacity on DPPH and ABTS radicals was observed in 30% acetone extract and was at 3 mg/mL for DPPH and 6 mg/mL for ABTS respectively. Ethanol extract showed the highest hydroxyl radical scavenging ability value of 66.36 at 20 mg/mL, followed by methanol (57.27), acetone (56.36) and water (30). The reducing potential of the different extracts was concentration dependent and increased with increase in concentration. These results indicate that substantial antioxidant activity can be obtained from Roselle seed phenolic compounds extract by using 30% acetone.
机译:这项研究的目的是从脱脂的玫瑰茄种子(Hibiscus sabdariffa L.)中提取酚类化合物并评估其抗氧化能力。水,乙醇(30%),甲醇(30%)和丙酮(30%)用作提取溶剂。分析了其最接近的成分,总酚含量和提取率。通过使用1,1-二苯基-2-辛基肼基(DPPH),羟基,2,2'-叠氮基双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除能力和还原能力来测试Roselle种子提取物的抗氧化功效分析。发现玫瑰茄种子富含蛋白质(27.745%),碳水化合物(40.45%)和油脂(20.83%)。分别使用水和30%丙酮时,总酚含量为1.66±0.03至1.99±0.01(GAE)mg / g。在30%丙酮提取物中观察到对DPPH和ABTS自由基的最高抑制能力,对DPPH的抑制作用为3 mg / mL,对于ABTS的抑制作用为6 mg / mL。乙醇提取物在20 mg / mL时具有最高的清除羟自由基能力,值为66.36,其次是甲醇(57.27),丙酮(56.36)和水(30)。不同提取物的还原电位取决于浓度,并且随着浓度的增加而增加。这些结果表明,通过使用30%丙酮,从玫瑰茄种子酚类化合物提取物中可以获得相当大的抗氧化活性。

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  • 来源
    《Advance journal of food science and technology》 |2013年第11期|1483-1489|共7页
  • 作者单位

    State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 Jiangsu, P.R. China;

    State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 Jiangsu, P.R. China;

    State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 Jiangsu, P.R. China;

    State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 Jiangsu, P.R. China;

    State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122 Jiangsu, P.R. China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Antioxidant activity; extraction solvent; proximate composition; roselle seed; total phenolic content;

    机译:抗氧化活性;萃取溶剂接近的组成;玫瑰茄种子;总酚含量;

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