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首页> 外文期刊>Advanced Functional Materials >Bright AlEgen-Protein Hybrid Nanocomposite for Deep and High-Resolution In Vivo Two-Photon Brain Imaging
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Bright AlEgen-Protein Hybrid Nanocomposite for Deep and High-Resolution In Vivo Two-Photon Brain Imaging

机译：明亮的AlEgen-蛋白质杂化纳米复合材料，可进行深度和高分辨率的体内双光子脑成像

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摘要

Two-photon fluorescence imaging allows in vivo study of biological structures and activities in deep tissues, in which bright fluorophores with high photostability and good biocompatibility are highly desirable. Herein, a small-molecule fluorogen with aggregation-induced emission (AIEgen) is complexed with fetal bovine serum (FBS) proteins to develop a protein-sized AIEgen-protein hybrid nanocomposite (TPEPy-FBS) with bright far-redear-infrared (NIR) emission, excellent photostability, and low phototoxicity for deep and high-resolution in vivo two-photon brain vasculature imaging. Upon complexation with FBS, the fluorescence of TPEPy is greatly intensified and a sixfold enhancement is observed with 10% FBS in aqueous media. The yielded TPEPy-FBS shows good physical stability in aqueous media and the phototoxicity of TPEPy is dramatically inhibited after complexation with FBS. Moreover, TPEPy-FBS exhibits bright two-photon fluorescence in far-red/NIR region and good photostability upon femtosecond laser excitation, which facilitates high performance in vivo imaging. A large imaging depth of 656 mu m is obtained in brain vasculature network imaging with a high signal-to-background ratio of 234, where a small blood capillary of 1.05 mu m can be resolved at an imaging depth of 656 mu m. Highlighted is a simple and versatile strategy to develop efficient two-photon probes for in vivo biological imaging.
机译：双光子荧光成像允许在体内研究深部组织的生物学结构和活性，其中高度期望具有高光稳定性和良好生物相容性的明亮荧光团。在此，将具有聚集诱导发射的小分子氟（AIEgen）与胎牛血清（FBS）蛋白复合，以开发出具有明亮远红/近红外光的蛋白大小的AIEgen-蛋白杂化纳米复合材料（TPEPy-FBS） （NIR）发射，出色的光稳定性和低光毒性，可进行深度和高分辨率的体内双光子脑血管成像。与FBS络合后，TPEPy的荧光大大增强，在水性介质中使用10％FBS观察到六倍的增强。所产生的TPEPy-FBS在水性介质中显示出良好的物理稳定性，并且与FBS络合后，TPEPy的光毒性被显着抑制。此外，TPEPy-FBS在远红/ NIR区域显示明亮的双光子荧光，并在飞秒激光激发后表现出良好的光稳定性，这促进了高性能的体内成像。在脑血管网络成像中获得了656μm的大成像深度，具有234的高信噪比，在656μm的成像深度下可以分辨出1.05μm的细毛细血管。重点是开发用于体内生物成像的高效两光子探针的简单且通用的策略。

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