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首页> 外文期刊>Advanced Functional Materials >Rotatable Aggregation-Induced-Emission/Aggregation- Caused-Quenching Ratio Strategy for Real-Time Tracking Nanoparticle Dynamics
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Rotatable Aggregation-Induced-Emission/Aggregation- Caused-Quenching Ratio Strategy for Real-Time Tracking Nanoparticle Dynamics

机译：实时跟踪纳米粒子动力学的可旋转聚集诱导发射/聚集引起的猝灭比策略

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摘要

Real-time tracking of the dynamics change of self-assembled nanostructures in physiological environments is crucial to improving their delivery efficiency and therapeutic effects. However, such tracking is impeded by the complex biological microenvironment leading to inhomogeneous distribution. A rotatable fluorescent ratio strategy is introduced that integrates aggregation-induced emission (AIE) and aggregation-caused quenching (ACQ) into one nanostructured system, termed AIE and ACQ fluorescence ratio (AAR). Following this strategy, an advanced probe, PEG(5k)-TPE4-ICGD(4) (PTI), is developed to track the dynamics change. The extremely sharp fluorescent changes (up to 4008-fold) in AAR allowed for the clear distinguishing and localization of the intact state and diverse dissociated states. The spatiotemporal distribution and structural dynamics of the PTI micelles can be tracked, quantitatively analyzed in living cells and animal tissue by the real-time ratio map, and be used to monitor other responsive nanoplatforms. With this method, the dynamics of nanoparticle in different organelles are able to be investigated and validated by transmission electron microscopy. This novel strategy is generally applicable to many self-assembled nanostructures for understanding delivery mechanism in living systems, ultimately to enhance their performance in biomedical applications.
机译：在生理环境中实时跟踪自组装纳米结构的动力学变化对于提高其递送效率和治疗效果至关重要。但是，复杂的生物微环境阻碍了这种跟踪，从而导致分布不均匀。引入了一种可旋转的荧光比率策略，该策略将聚集诱导发射（AIE）和聚集引起的猝灭（ACQ）集成到一个纳米结构系统中，称为AIE和ACQ荧光比率（AAR）。按照此策略，开发了一种先进的探针PEG（5k）-TPE4-ICGD（4）（PTI），以跟踪动力学变化。 AAR中非常尖锐的荧光变化（高达4008倍）可以使完整状态和各种解离状态得到清晰的区分和定位。可以追踪PTI胶束的时空分布和结构动力学，并通过实时比率图在活细胞和动物组织中进行定量分析，并将其用于监测其他响应性纳米平台。使用这种方法，可以通过透射电子显微镜研究和验证纳米颗粒在不同细胞器中的动力学。这种新颖的策略通常适用于许多自组装的纳米结构，以了解生命系统中的传递机制，最终提高其在生物医学应用中的性能。

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