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首页> 外文期刊>Advanced Functional Materials >Void-Free 3D Bioprinting for In Situ Endothelialization and Microfluidic Perfusion
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Void-Free 3D Bioprinting for In Situ Endothelialization and Microfluidic Perfusion

机译：无空隙3D生物打印用于原位内皮化和微流灌注

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摘要

Two major challenges of 3D bioprinting are the retention of structural fidelity and efficient endothelialization for tissue vascularization. Both of these issues are addressed by introducing a versatile 3D bioprinting strategy, in which a templating bioink is deposited layer-by-layer alongside a matrix bioink to establish void-free multimaterial structures. After crosslinking the matrix phase, the templating phase is sacrificed to create a well-defined 3D network of interconnected tubular channels. This void-free 3D printing (VF-3DP) approach circumvents the traditional concerns of structural collapse, deformation, and oxygen inhibition, moreover, it can be readily used to print materials that are widely considered "unprintable." By preloading endothelial cells into the templating bioink, the inner surface of the channels can be efficiently cellularized with a confluent endothelial layer. This in situ endothelialization method can be used to produce endothelium with a far greater cell seeding uniformity than can be achieved using the conventional postseeding approach. This VF-3DP approach can also be extended beyond tissue fabrication and toward customized hydrogel-based microfluidics and self-supported perfusable hydrogel constructs.
机译：3D生物打印的两个主要挑战是保留结构保真度和有效的内皮化以实现组织血管化。通过引入一种通用的3D生物打印策略可以解决这两个问题，在该策略中，将模板生物墨水与基质生物墨水一起逐层沉积，以建立无空隙的多材料结构。交联基体相后，牺牲模板相以创建相互连接的管状通道的定义明确的3D网络。这种无空隙的3D打印（VF-3DP）方法规避了结构塌陷，变形和氧气抑制的传统问题，此外，它还可以轻松地用于打印被广泛认为“不可打印”的材料。通过将内皮细胞预加载到模板生物墨水中，通道的内表面可以被融合的内皮层有效地细胞化。这种原位内皮化方法可用于产生具有比常规后种方法更高的细胞播种均匀性的内皮。这种VF-3DP方法还可以扩展到组织制造之外，还可以扩展到基于水凝胶的定制微流控技术和可自支撑的可灌输水凝胶构造。

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