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首页> 外文期刊>Advanced Functional Materials >Induction of Four-Dimensional Spatiotemporal Geometric Transformations in High Cell Density Tissues via Shape-Changing Hydrogels
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Induction of Four-Dimensional Spatiotemporal Geometric Transformations in High Cell Density Tissues via Shape-Changing Hydrogels

机译：通过形状改变水凝胶诱导高细胞密度组织中的四维时空几何变换

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摘要

Developing and healing tissues begin as cellular condensations. Spatiotemporal changes in tissue geometry, transformations in the spatial distribution of the cells, and extracellular matrix are essential for its evolution into a functional tissue. 4D materials, 3D materials capable of geometric changes, may have the potential to recreate the aforementioned biological phenomenon. However, most reported 4D materials are non-degradable and/or not biocompatible, limiting their application in regenerative medicine, and to date, there are no systems controlling the geometry of high density cellular condensations and differentiation. Here, 4D high cell density tissues based on shape-changing hydrogels are described. By sequential photocrosslinking of oxidized and methacrylated alginate (OMA) and methacrylated gelatin (GelMA), bilayered hydrogels presenting controllable geometric changes without any external stimuli are fabricated. Fibroblasts and human adipose-derived stem cells (ASCs) are encapsulated at concentrations up to 1.0 x 10(8) cells mL(-1) in the 4D constructs, and controllable shape changes are achieved in concert with ASCs differentiated down chondrogenic and osteogenic lineages. Bioprinting of the high density cell-laden OMA and GelMA permits the formation of more complex constructs with defined 4D geometric changes, which may further expand the promise of this approach in regenerative medicine applications.
机译：发展和愈合组织开始作为蜂窝缩合。组织几何形状的时空变化，细胞的空间分布中的转化，细胞外基质对于其进化至功能组织至关重要。 4D材料，3D材料能够进行几何变化，可能有可能重新创建上述生物现象。然而，大多数报道的4D材料是不可降解的和/或不是生物相容性，限制其在再生医学中的应用，并且迄今为止，没有系统控制高密度细胞缩合和分化的几何形状。这里，描述了基于形状改变水凝胶的4D高细胞密度组织。通过氧化和甲基丙烯酸丙酸丙烯酸盐（OMA）和甲基丙烯酸明胶（GELMA）的顺序光源，呈现可控制的几何变化而没有任何外部刺激的双层水凝胶。成纤维细胞和人脂肪衍生的干细胞（ASCS）在4D构建体中浓缩至1.0×10（8）个细胞ml（-1）的浓度，并且在鉴别的心电图和骨质发生谱系中，Concent实现可控形状变化。高密度电池覆盖OMA和GELMA的生物印刷允许形成具有定义的4D几何变化的更复杂构建体，这可能进一步扩大这种方法在再生医学应用中的承诺。

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