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Fish-Microarray: A Miniaturized Platform for Single-Embryo High-Throughput Screenings

机译：鱼微阵列：用于单胚胎高通量筛选的小型化平台

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摘要

Small molecule high-throughput screenings are essential for the fields of drug discovery and toxicology. Such screenings performed on whole animals are more physiologically relevant leading to more predictive results. However, due to challenges in automation, high costs and absence of miniaturized solutions for animal-based experiments, high throughput screenings based on animal models are still in its infancy. Here a platform for miniaturized high throughput whole-organism screenings is presented. The new platform is based on patterns of hydrophilic spots separated by superhydrophobic borders. The difference in wettability of spots and borders generates the effect of discontinuous dewetting and allows for formation of arrays of microdroplets that incorporate single fish embryos. Due to the flat border-less nature of the platform, the system is compatible with single-step collection of embryos and pipetting-free parallel addition of chemical libraries using the "sandwiching method." The system is miniaturized and allows for incubation of embryos in volumes as low as 5 mu L.. Finally, the platform realizes surface tension based immobilization of single embryos inside of each microcompartment and permits high-throughput microscopic analysis directly on the platform. Thus, this method combines the advantages of microarrays, such as high-throughput and simplicity, with the power of in vivo experiments.
机译：小分子高通量筛选对于药物发现和毒理学领域至关重要。对整只动物进行的此类筛查在生理上更具相关性，从而导致更具预测性的结果。然而，由于自动化方面的挑战，高成本以及缺乏基于动物的实验的小型化解决方案，基于动物模型的高通量筛选仍处于起步阶段。这里介绍了一个用于小型高通量全生物筛选的平台。新平台基于由超疏水性边界分隔的亲水性斑点的图案。点和边界的可湿性差异产生不连续的去湿效果，并允许形成包含单个鱼胚的微滴阵列。由于平台具有平坦的无边界特性，因此该系统与胚胎的单步收集以及使用“夹心法”的无吸液并行添加化学库兼容。该系统已小型化，可用于孵化低至5μL的胚胎。最后，该平台实现了基于表面张力的单个微隔室内单个胚胎的固定化，并允许直接在平台上进行高通量显微镜分析。因此，该方法结合了微阵列的优势，例如高通量和简便性，以及体内实验的强大功能。

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  • 来源
    《Advanced Functional Materials》 |2018年第3期|1703486.1-1703486.12|共12页
  • 作者

    Popova Anna A.; Marcato Daniel; Peravali Ravindra; Wehl Ilona; Schepers Ute; Levkin Pavel A.;

  • 作者单位

    KIT, Inst Toxicol & Genet, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany;

    KIT, Inst Toxicol & Genet, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany;

    KIT, Inst Toxicol & Genet, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany;

    KIT, Inst Toxicol & Genet, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany;

    KIT, Inst Toxicol & Genet, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany|KIT, Inst Organ Chem, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany;

    KIT, Inst Toxicol & Genet, Hermann von Helmholtz Pl 1, D-76344 Eggenstein Leopoldshafen, Germany|KIT, Inst Organ Chem, Fritz Haber Weg 6, D-76131 Karlsruhe, Germany;

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  • 正文语种 eng
  • 中图分类
  • 关键词

    Danio rerio (zebrafish); droplet-microarrays; patterning; screening; superhydrophobicity; whole-organism screening;

    机译：斑马鱼（Danio rerio）（斑马鱼）;液滴微阵列;构图;筛选;超疏水性;全生物筛选;

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