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首页> 外文期刊>Advanced Functional Materials >Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering
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Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering

机译：电场辅助微流体平台，可产生可定制的多孔微珠作为组织工程的细胞载体

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摘要

Injection of cell-laden scaffolds in the form of mesoscopic particles directly to the site of treatment is one of the most promising approaches to tissue regeneration. Here, a novel and highly efficient method is presented for preparation of porous microbeads of tailorable dimensions (in the range approximate to 300-1500 mu m) and with a uniform and fully interconnected internal porous texture. The method starts with generation of a monodisperse oil-in-water emulsion inside a flow-focusing microfluidic device. This emulsion is later broken-up, with the use of electric field, into mesoscopic double droplets, that in turn serve as a template for the porous microbeads. By tuning the amplitude and frequency of the electric pulses, the template droplets and the resulting porous bead scaffolds are precisely produced. Furthermore, a model of pulsed electrodripping is proposed that predicts the size of the template droplets as a function of the applied voltage. To prove the potential of the porous microbeads as cell carries, they are tested with human mesenchymal stem cells and hepatic cells, with their viability and degree of microbead colonization being monitored. Finally, the presented porous microbeads are benchmarked against conventional microparticles with nonhomogenous internal texture, revealing their superior performance.
机译：将介孔颗粒形式的充满细胞的支架直接注射到治疗部位是组织再生的最有希望的方法之一。在此，提出了一种新颖且高效的方法，用于制备可定制尺寸（范围约为300-1500μm）且具有均匀且完全互连的内部多孔质感的多孔微珠。该方法开始于在流动聚焦微流体装置内部产生单分散的水包油乳液。该乳液随后在电场作用下分解为介观双液滴，进而用作多孔微珠的模板。通过调节电脉冲的幅度和频率，可以精确地产生模板液滴和所得的多孔珠支架。此外，提出了一种脉冲电滴模型，该模型预测了模板液滴的大小与所施加电压的关系。为了证明多孔微珠作为细胞携带的潜力，将它们与人间充质干细胞和肝细胞进行了测试，并监测了它们的生存力和微珠定植程度。最后，提出的多孔微珠与具有非均质内部质地的常规微粒进行了基准比较，揭示了它们的优越性能。

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