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Selective Photothermal Tumor Therapy Using Nanodiamond-Based Nanoclusters with Folic Acid

机译：基于纳米金刚石的纳米簇与叶酸的选择性光热肿瘤治疗

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摘要

This paper describes the fabrication and evaluation of folic acid (FA)-conjugated nanodiamond (ND) nanoclusters for selective photothermal tumor therapy. ND nanoclusters with surface carboxyl groups are aminated using ethylenediamine and conjugated with FA via carbodiimide chemistry. The temperature of an aqueous ND dispersion (10 mu g mL(-1)) is increased to 54 degrees C upon laser exposure for 5 min. FA-ND nanoclusters are preferentially taken up by KB cells (folate receptor positive) compared to WI-38 (folate receptor negative) cells, suggesting specificity for tumor cells that overexpress folate receptors. Cell viability tests reveal that FA-ND nanoclusters effectively and selectively ablate KB cells upon near-infrared (NIR) laser exposure. In addition, fluorescence microscopy images confirm that only KB cells treated with FA-ND nanoclusters are ablated in a spot (200 mu m in diameter) by NIR laser exposure. In an animal model, a large amount of FA-ND nanoclusters is accumulated into tumor tissue, resulting in dramatically reduced tumor volume post-NIR laser exposure as compared to ND nanoclusters.
机译：本文描述了选择性光热肿瘤治疗中叶酸（FA）-共轭纳米金刚石（ND）纳米簇的制备和评价。使用乙二胺胺化具有表面羧基的ND纳米簇，并通过碳二亚胺化学法将FA与FA偶联。激光曝光5分钟后，ND水分散液（10μg mL（-1））的温度升至54摄氏度。与WI-38（叶酸受体阴性）细胞相比，FA-ND纳米簇优先被KB细胞（叶酸受体阳性）吸收，这表明对过表达叶酸受体的肿瘤细胞具有特异性。细胞活力测试表明，FA-ND纳米团簇可在近红外（NIR）激光照射下有效，选择性地烧蚀KB细胞。此外，荧光显微镜图像证实，仅通过FA-ND纳米簇处理的KB细胞通过NIR激光照射在一个点（直径200微米）中被烧蚀。在动物模型中，大量的FA-ND纳米团簇积聚在肿瘤组织中，与ND纳米团簇相比，NIR激光暴露后肿瘤体积大大减少。

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  • 来源
    《Advanced Functional Materials》 |2016年第35期|6428-6436|共9页
  • 作者

    Ryu Tae-Kyung; Baek Seung Woon; Kang Rae Hyung; Choi Sung-Wook;

  • 作者单位

    Catholic Univ Korea, Dept Biotechnol, 43 Jibong Ro, Bucheon Si 420743, Gyeonggi Do, South Korea;

    Catholic Univ Korea, Dept Biotechnol, 43 Jibong Ro, Bucheon Si 420743, Gyeonggi Do, South Korea;

    Catholic Univ Korea, Dept Biotechnol, 43 Jibong Ro, Bucheon Si 420743, Gyeonggi Do, South Korea;

    Catholic Univ Korea, Dept Biotechnol, 43 Jibong Ro, Bucheon Si 420743, Gyeonggi Do, South Korea;

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