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首页> 外文期刊>Advanced Functional Materials >Tunable Nanoparticle and Cell Assembly Using Combined Self-Powered Microfluidics and Microcontact Printing
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Tunable Nanoparticle and Cell Assembly Using Combined Self-Powered Microfluidics and Microcontact Printing

机译：结合自供电微流控和微接触印刷的可调谐纳米粒子和细胞组装。

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摘要

The combination of cell microenvironment control and real-time monitoring of cell signaling events can provide key biological information. Through precise multipatterning of gold nanoparticles (GNPs) around cells, sensing and actuating elements can be introduced in the cells' microenviroment, providing a powerful substrate for cell studies. In this work, a combination of techniques are implemented to engineer complex substrates for cell studies. Alternating GNPs and bioactive areas are created with micrometer separation by means of a combination of vacumm soft-lithography of GNPs and protein microcontract printing. Instead of conventional microfluidics that need syringe pumps to flow liquid in the microchannels, degas driven flow is used to fill dead-end channels with GNP solutions, rendering the fabrication process straightforward and accessible. This new combined technique is called Printing and Vacuum lithography (PnV lithography). By using different GNPs with various organic coating ligands, different macroscale patterns are obtained, such as wires, supercrystals, and uniformly spread nanoparticle layers that can find different applications depending on the need of the user. The application of the system is tested to pattern a range of mammalian cell lines and obtain readouts on cell viability, cell morphology, and the presence of cell adhesive proteins.
机译：细胞微环境控制与细胞信号事件实时监控的结合可以提供关键的生物学信息。通过在细胞周围对金纳米颗粒（GNP）进行精确的多图案化，可以将传感和驱动元件引入细胞的微环境中，为细胞研究提供强大的基质。在这项工作中，实现了多种技术组合以工程化用于细胞研究的复杂底物。交替的GNP和生物活性区域是通过真空分离GNP的真空光刻技术和蛋白质微合同印刷的组合，通过千分尺分离而创建的。代替需要注射泵使液体在微通道中流动的常规微流体，脱气驱动的流量用于通过GNP解决方案填充死角通道，从而使制造过程变得简单易行。这种新的组合技术称为印刷和真空光刻（PnV光刻）。通过将不同的GNP与各种有机涂层配体一起使用，可以获得不同的宏观图案，例如导线，超晶和均匀分布的纳米颗粒层，这些层可以根据用户的需求找到不同的应用。该系统的应用经过测试，可以对一系列哺乳动物细胞系进行模式分析，并获得有关细胞活力，细胞形态和细胞粘附蛋白存在的读数。

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  • 来源
    《Advanced Functional Materials》 |2016年第44期|8053-8061|共9页
  • 作者

    Hamon Cyrille; Henriksen-Lacey Malou; La Porta Andrea; Rosique Melania; Langer Judith; Scarabelli Leonardo; Serrano Montes Ana Belen; Gonzalez-Rubio Guillermo; de Pancorbo Marian M.; Liz-Marzan Luis M.; Basabe-Desmonts Lourdes;

  • 作者单位

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain;

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain|CIBER BBN, Madrid 28029, Spain;

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain;

    Univ Basque Country UPV EHU, Lascaray Ikergunea Res Ctr, BIOM Res Grp, Vitoria 01006, Spain;

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain;

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain;

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain;

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain;

    Univ Basque Country UPV EHU, Lascaray Ikergunea Res Ctr, BIOM Res Grp, Vitoria 01006, Spain;

    CIC BiomaGUNE, Bionanoplasmon Lab, Donostia San 20009, Sebastian, Spain|CIBER BBN, Madrid 28029, Spain|Basque Fdn Sci, Ikerbasque, Bilbao 48013, Spain;

    Univ Basque Country UPV EHU, Lascaray Ikergunea Res Ctr, BIOM Microfluid Res Grp, Microfluid Cluster UPV EHU, Vitoria 01006, Spain|Basque Fdn Sci, Ikerbasque, Bilbao 48013, Spain;

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