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首页> 外文期刊>Advanced Functional Materials >Topological Effects on Globular Protein-ELP Fusion Block Copolymer Self-Assembly
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Topological Effects on Globular Protein-ELP Fusion Block Copolymer Self-Assembly

机译：球形蛋白-ELP融合嵌段共聚物自组装的拓扑效应

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摘要

Perfectly defined, monodisperse fusion protein block copolymers of a thermoresponsive coil-like protein, ELP, and a globular protein, mCherry, are demonstrated to act as fully biosynthetic analogues to protein-polymer conjugates that can self-assemble into biofunctional nanostructures such as hexagonal and lamellar phases in concentrated solutions. The phase behavior of two mCherry-ELP fusions, E_(10)-mCherry-E_(10) and E_(20)-mCherry, is investigated to compare linear and bola fusion self-assembly both in diluted and concentrated aqueous solution. In dilute solution, the molecular topology impacts the stability of micelles formed above the thermal transition temperature of the ELP block, with the diblock forming micelles and the bola forming unstable aggregates. Despite the chemical similarity of the two protein blocks, the materials order into block copolymer-like nanostructures across a wide range of concentrations at 30 wt% and above, with the bola fusion having a lower order-disorder transition concentration than the diblock fusion. The topology of the molecule has a large impact on the type of nano-structure formed, with the two fusions forming phases in the opposite order as a function of temperature and concentration. This new system provides a rich landscape to explore the capabilities of fusion architecture to control supramolecular assemblies for bioactive materials.
机译：完美定义的热响应性线圈状蛋白ELP和球状蛋白mCherry的单分散融合蛋白嵌段共聚物可作为蛋白质-聚合物共轭物的完全生物合成类似物，后者可自组装成生物功能纳米结构，例如六角形和浓溶液中的层状相。研究了两种mCherry-ELP融合体E_（10）-mCherry-E_（10）和E_（20）-mCherry的相行为，以比较稀释和浓缩水溶液中的线性和bola融合自组装。在稀溶液中，分子拓扑会影响在ELP嵌段的热转变温度以上形成的胶束的稳定性，其中双嵌段形成胶束，而玻拉形成不稳定的聚集体。尽管两个蛋​​白质嵌段在化学上相似，但该材料在30 wt％或更高的宽浓度范围内仍排列成嵌段共聚物样纳米结构，其中bola融合蛋白的有序-无序过渡浓度低于双嵌段融合物。分子的拓扑结构对所形成的纳米结构的类型有很大的影响，两次融合形成的相依温度和浓度的不同而相反。这个新系统为探索融合架构控制生物活性材料的超分子组装提供了广阔的前景。

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  • 来源
    《Advanced Functional Materials》 |2015年第5期|729-738|共10页
  • 作者

    Guokui Qin; Matthew J. Glassman; Christopher N. Lam; Dongsook Chang; Eric Schaible; Alexander Hexemer; Bradley D. Olsen;

  • 作者单位

    Department of Chemical Engineering Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge, MA 02139, USA;

    Department of Chemical Engineering Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge, MA 02139, USA;

    Department of Chemical Engineering Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge, MA 02139, USA;

    Department of Chemical Engineering Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge, MA 02139, USA;

    Advanced Light Source Lawrence Berkeley National Laboratory 1 Cyclotron Road, Berkeley, CA 94720, USA;

    Advanced Light Source Lawrence Berkeley National Laboratory 1 Cyclotron Road, Berkeley, CA 94720, USA;

    Department of Chemical Engineering Massachusetts Institute of Technology 77 Massachusetts Avenue Cambridge, MA 02139, USA;

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