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Magnetic Helical Microswimmers Functionalized with Lipoplexes for Targeted Gene Delivery

机译：脂质复合物功能化的电磁螺旋微泳蛋白用于靶向基因传递。

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摘要

Artificial micro-anoswimmers have various potential applications including minimally invasive diagnosis and targeted therapies, environmental sensing and monitoring, cell manipulation and analysis, and lab-on-a-chip devices. Inspired by natural motile bacteria such as E. Coli, artificial bacterial flagella (ABFs) are one kind of magnetic helical microswimmers. ABFs can perform 3D navigation in a controllable fashion with micrometer precision under low-strength rotating magnetic fields (＜10 mT) and are promising tools for targeted drug delivery in vitro and in vivo. In this work, the successful wirelessly targeted and single-cell gene delivery to human embryonic kidney (HEK 293) cells using ABFs loaded with plasmid DNA (pDNA) in vitro is demonstrated for the first time. The ABFs are functionalized with lipoplexes containing pDNA to generate functionalized ABFs (f-ABFs). The f-ABFs are steered wirelessly by low-strength rotating magnetic fields and deliver the loaded pDNA into targeted cells. The cells targeted by f-ABFs are successfully transfected by the transported pDNA and expressed the encoding protein. These f-ABFs may also be useful for in vivo gene delivery and other applications such as sensors, actuators, cell biology, and lab-on-a-chip environments.
机译：人工微/纳米洗涤器具有各种潜在的应用，包括微创诊断和靶向疗法，环境传感和监测，细胞操纵和分析以及芯片实验室设备。受到大肠杆菌等自然运动细菌的启发，人工鞭毛（ABF）是一种电磁螺旋微游泳器。 ABF可以在低强度旋转磁场（＜10 mT）下以微米级的精度以可控的方式执行3D导航，并且是用于体外和体内靶向药物递送的有前途的工具。在这项工作中，首次证明了使用载有质粒DNA（pDNA）的ABF在体外成功地将无线靶向单细胞基因递送至人胚胎肾（HEK 293）细胞。用含有pDNA的脂质复合物对ABF进行功能化，以生成功能化的ABF（f-ABF）。 f-ABFs通过低强度旋转磁场进行无线控制，并将装载的pDNA输送到目标细胞中。 f-ABFs靶向的细胞被转运的pDNA成功转染并表达了编码蛋白。这些f-ABF还可用于体内基因传递和其他应用，例如传感器，致动器，细胞生物学和芯片实验室环境。

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  • 来源
    《Advanced Functional Materials》 |2015年第11期|1666-1671|共6页
  • 作者

    Famin Qiu; Satoshi Fujita; Rami Mhanna; Li Zhang; Benjamin R. Simona; Bradley J. Nelson;

  • 作者单位

    Institute of Robotics and Intelligent System (IRIS) ETH Zurich, Zurich CH-8092, Switzerland;

    Biomedical Research Institute AIST, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan,Laboratory of Biosensors and Bioelectronics University and ETH Zurich Zurich CH-8092, Switzerland;

    Biomedical Engineering American University of Beirut Beirut 1107 2020, Lebanon;

    Department of Mechanical and Automation Engineering The Chinese University of Hong Kong Hong Kong SAR, China,Chow Yuk Ho Technology Centre for Innovative Medicine The Chinese University of Hong Kong Hong Kong SAR, China;

    Laboratory of Biosensors and Bioelectronics University and ETH Zurich Zurich CH-8092, Switzerland;

    Institute of Robotics and Intelligent System (IRIS) ETH Zurich, Zurich CH-8092, Switzerland;

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