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首页> 外文期刊>Advanced Functional Materials >Oxidized Porous Silicon Nanostructures Enabling Electrokinetic Transport for Enhanced DNA Detection
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Oxidized Porous Silicon Nanostructures Enabling Electrokinetic Transport for Enhanced DNA Detection

机译：氧化多孔硅纳米结构使电动传输增强DNA检测。

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摘要

Nanostructured porous silicon (PSi) is a promising material for the label-free detection of biomolecules, but it currently suffers from limited applicability due to poor sensitivity, typically in micromolar range. This work presents the design, operation concept, and characterization of a novel microfluidic device and assay that integrates an oxidized PSi optical biosensor with electrokinetic focusing for a highly sensitive label-free detection of nucleic acids. Under proper oxidation conditions, the delicate nanostructure of PSi can be preserved, while providing sufficient dielectric insulation for application of high voltages. This enables the use of signal enhancement techniques, which are based on electric fields. Here, the DNA target molecules are focused using an electric field within a finite and confined zone, and this highly concentrated analyte is delivered to an on-chip PSi Fabry-Perot optical transducer, pre-functionalized with capture probes. Using reflective interferometric Fourier transform spectroscopy real-time monitoring, a 1000-fold improvement in limit of detection is demonstrated compared to a standard assay, using the same biosensor. Thus, a measured limit of detection of 1 x 10(-9) M is achieved without compromising specificity. The concepts presented herein can be readily applied to other ionic targets, paving way for the development of other highly sensitive chemical and biochemical assays.
机译：纳米结构多孔硅（PSi）是用于无标记生物分子检测的有前途的材料，但是由于灵敏度低（通常在微摩尔范围内），目前它的适用性受到限制。这项工作提出了一种新型的微流体装置和测定的设计，操作概念和表征，该装置将氧化的PSi光学生物传感器与电动聚焦相结合，可实现高度灵敏的无标记核酸检测。在适当的氧化条件下，可以保留PSi的精细纳米结构，同时为施加高压提供足够的介电绝缘。这使得能够使用基于电场的信号增强技术。在这里，DNA靶分子在有限的封闭区域内使用电场聚焦，然后将这种高度浓缩的分析物输送到芯片上的PSi Fabry-Perot光学传感器，该传感器预先用捕获探针进行了功能化。使用反射式干涉傅里叶变换光谱实时监控，与使用相同生物传感器的标准测定法相比，检测限提高了1000倍。因此，在不影响特异性的情况下，可实现1 x 10（-9）M的检测极限。本文提出的概念可以容易地应用于其他离子目标，为开发其他高度敏感的化学和生物化学测定法铺平了道路。

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  • 来源
    《Advanced Functional Materials》 |2015年第43期|6725-6732|共8页
  • 作者

    Vilensky Rita; Bercovici Moran; Segal Ester;

  • 作者单位

    Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-3200003 Haifa, Israel;

    Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-3200003 Haifa, Israel|Technion Israel Inst Technol, Fac Mech Engn, IL-3200003 Haifa, Israel;

    Technion Israel Inst Technol, Russell Berrie Nanotechnol Inst, IL-3200003 Haifa, Israel|Technion Israel Inst Technol, Fac Biotechnol & Food Engn, IL-3200003 Haifa, Israel;

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