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首页> 外文期刊>Advanced Functional Materials >Iron Oxide-Labeled Collagen Scaffolds for Non-Invasive MR Imaging in Tissue Engineering
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Iron Oxide-Labeled Collagen Scaffolds for Non-Invasive MR Imaging in Tissue Engineering

机译：氧化铁标记的胶原蛋白支架用于组织工程中的无创MR成像

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摘要

Non-invasive imaging holds significant potential for implementation in tissue engineering. It can be used to monitor the localization and function of tissue-engineered implants, as well as their resorption and remodelling. Thus far, however, the vast majority of effort in this area of research have focused on the use of ultrasmall super-paramagnetic iron oxide (USPIO) nanoparticle-labeled cells, colonizing the scaffolds, to indirectly image the implant material. Reasoning that directly labeling scaffold materials might be more beneficial (enabling imaging also in the case of non-cellularized implants), more informative (enabling the non-invasive visualization and quantification of scaffold degradation), and easier to translate into the clinic (cell-free materials are less complex from a regulatory point-of-view), three different types of USPIO nanoparticles are prepared and incorporated both passively and actively (via chemical conjugation; during collagen crosslinking) into collagen-based scaffold materials. The amount of USPIO incorporated into the scaffolds is optimized, and correlated with MR signal intensity, showing that the labeled scaffolds are highly biocompatible, and that scaffold degradation can be visualized using MRI. This provides an initial proof-of-principle for the in vivo visualization of the scaffolds. Consequently, USPIO-labeled scaffold materials seem to be highly suitable for image-guided tissue engineering applications.
机译：非侵入性成像在组织工程中具有巨大的实施潜力。它可以用于监视组织工程植入物的定位和功能，以及它们的吸收和重塑。然而，迄今为止，在该研究领域中的绝大多数努力都集中在使用超小型超顺磁性氧化铁（USPIO）纳米颗粒标记的细胞上，该细胞定殖在支架上，以间接使植入物成像。认为直接标记支架材料可能会更有利（在非细胞化植入物的情况下也可以进行成像），提供更多信息（可以对支架降解进行非侵入式可视化和量化），并且更易于转化为临床（细胞从监管的角度来看，游离的材料不太复杂），制备了三种不同类型的USPIO纳米颗粒，并将它们被动和主动（通过化学偶联；在胶原蛋白交联过程中）掺入到基于胶原蛋白的支架材料中。优化了掺入支架的USPIO的量，并将其与MR信号强度相关联，表明标记的支架具有高度的生物相容性，并且可以使用MRI观察支架的降解情况。这为支架的体内可视化提供了初步的原理证明。因此，USPIO标记的支架材料似乎非常适合图像引导的组织工程应用。

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  • 来源
    《Advanced Functional Materials》 |2014年第6期|754-762|共9页
  • 作者

    Marianne E. Mertens; Alina Hermann; Anne Buehren; Leon Olde-Damink; Diana Moeckel; Felix Gremse; Josef Ehling; Fabian Kiessling; Twan Lammers;

  • 作者单位

    Department for Experimental Molecular Imaging University Clinic and Helmholtz Institute for Biomedical Engineering RWTH -Aachen University Pauwelsstrasse 20, 52074, Aachen, Germany;

    Matricel GmbH Kaiserstrasse 100, 52134, Herzogenrath, Germany;

    Matricel GmbH Kaiserstrasse 100, 52134, Herzogenrath, Germany;

    Matricel GmbH Kaiserstrasse 100, 52134, Herzogenrath, Germany;

    Department for Experimental Molecular Imaging University Clinic and Helmholtz Institute for Biomedical Engineering RWTH -Aachen University Pauwelsstrasse 20, 52074, Aachen, Germany;

    Department for Experimental Molecular Imaging University Clinic and Helmholtz Institute for Biomedical Engineering RWTH -Aachen University Pauwelsstrasse 20, 52074, Aachen, Germany;

    Department for Experimental Molecular Imaging University Clinic and Helmholtz Institute for Biomedical Engineering RWTH -Aachen University Pauwelsstrasse 20, 52074, Aachen, Germany;

    Department for Experimental Molecular Imaging University Clinic and Helmholtz Institute for Biomedical Engineering RWTH -Aachen University Pauwelsstrasse 20, 52074, Aachen, Germany;

    Department for Experimental Molecular Imaging University Clinic and Helmholtz Institute for Biomedical Engineering RWTH -Aachen University Pauwelsstrasse 20, 52074, Aachen, Germany,Department of Controlled Drug Delivery MIRA Institute for Biomedical Engineering and Technical Medicine University of Twente PO Box 217, 7500, AE, Enschede, The Netherlands;

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