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首页> 外文期刊>Advanced Functional Materials >Tenogenic Induction of Human MSCs by Anisotropically Aligned Collagen Biotextiles
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Tenogenic Induction of Human MSCs by Anisotropically Aligned Collagen Biotextiles

机译：各向异性排列的胶原生物纺织品对人间充质干细胞的张力诱导。

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摘要

A novel biofabrication modality, electrophoretic compaction with macro-molecular alignment, is utilized to make collagen threads that mimic the native tendon's structure and mechanical properties. A device with kinematic electrodes is designed to fabricate collagen threads in continuous length. For the first time, a 3D-biotextile is woven purely from collagen. Mechanical properties and load-displacement behavior of the biotextile mimic those of the native tendon while presenting a porosity of 80%. The open pore network facilitates cell seeding across the continuum of the bioscaffold. Mesenchymal stem cells (MSCs) seeded in the woven scaffold undergo tenogenic differentiation in the absence of growth factors and synthesize a matrix that is positive for tenomodulin, COMP and type Ⅰ collagen. Up-regulation of tenomodulin, a tendon specific marker, is 11.6 ± 3.5 fold, COMP is up-regulated 16.7 ± 5.5 fold, and Col I is up-regulated 6.9 ± 2.7 fold greater on ELAC threads when compared to randomly oriented collagen gels. These results demonstrate that a bioscaffold woven using collagen threads with densely compacted and anisotropically aligned substrate texture stimulates tenogenesis topographically, rendering the electrochemically aligned collagen as a promising candidate for functional repair of tendons and ligaments.
机译：一种新型的生物制造方式，即具有大分子排列的电泳压实技术，可用于制造模仿天然肌腱结构和机械性能的胶原蛋白线。具有运动电极的设备被设计为制造连续长度的胶原线。第一次，3D生物纺织品仅由胶原蛋白编织而成。生物织物的机械性能和负荷位移行为模仿了天然肌腱的力学性能和负荷位移行为，同时孔隙率为80％。开孔网络有助于跨整个生物支架的细胞播种。接种在编织支架中的间充质干细胞（MSCs）在没有生长因子的情况下进行肌腱分化，并合成了一个对Tenomodulin，COMP和Ⅰ型胶原呈阳性的基质。与随机定向的胶原蛋白凝胶相比，肌腱特异性标志物Tenomodulin的上调为11.6±3.5倍，COMP的上调为16.7±5.5倍，Col I的上调为ELAC螺纹上的6.9±2.7倍。这些结果表明，使用胶原线编织的生物支架具有紧密压实的各向异性排列的底物纹理，在地形上刺激腱鞘形成，使电化学排列的胶原成为腱和韧带功能修复的有前途的候选者。

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  • 来源
    《Advanced Functional Materials》 |2014年第36期|5762-5770|共9页
  • 作者

    Mousa Younesi; Anowarul Islam; Vipuil Kishore; James M. Anderson; Ozan Akkus;

  • 作者单位

    Department of Mechanical and Aerospace Engineering Case Western Reserve University Cleveland, OH 44106, USA;

    Department of Mechanical and Aerospace Engineering Case Western Reserve University Cleveland, OH 44106, USA;

    Department of Chemical Engineering Florida Institute of Technology Melbourne, FL 32901, USA;

    Department of Pathology Case Western Reserve University Cleveland, OH 44106, USA,Department of Macromolecular Science Case Western Reserve University Cleveland, OH 44106, USA,Department of Biomedical Engineering Case Western Reserve University Cleveland, OH 44106, USA;

    Department of Mechanical and Aerospace Engineering Case Western Reserve University Cleveland, OH 44106, USA,Department of Orthopedics Case Western Reserve University Cleveland, OH 44106, USA,Department of Biomedical Engineering Case Western Reserve University Cleveland, OH 44106, USA;

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