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首页> 外文期刊>Advanced Functional Materials >Catalytic Saloplastics: Alkaline Phosphatase Immobilized and Stabilized in Compacted Polyelectrolyte Complexes
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Catalytic Saloplastics: Alkaline Phosphatase Immobilized and Stabilized in Compacted Polyelectrolyte Complexes

机译：催化的合成油塑剂：碱性磷酸酶固定并稳定在压实的聚电解质复合物中

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摘要

Novel biochemically active compact polyelectrolyte complexes (CoPECs) are obtained through a simple coprecipitation and compaction procedure. As shown for the system composed of poly (acrylic acid) (PAA) and poly(allylamine) (PAH) as polyelectrolytes and alkaline phosphatase (ALP) as enzyme, the enzyme can be firmly immobilized into these materials. The ALP not only remains active in these materials, but the matrix also enhances the specific activity of the enzyme while protecting it from deactivation at higher temperature. The presence of the matrix allows fine control and substantial enhancement of reaction rates by varying the salt concentration of the contacting solution or temperature. The excellent reusability, together with the ease of co-immobilizing other useful components, such as magnetic particles, allowing facile handling of the CoPECs, makes these materials interesting candidates for variable scaffolds for the immobilization of enzymes for small-and large-scale enzyme-catalyzed processes.
机译：通过简单的共沉淀和压实程序可获得新型的生化活性紧密聚电解质复合物（CoPEC）。如由聚（丙烯酸）（PAA）和聚（烯丙胺）（PAH）作为聚电解质和碱性磷酸酶（ALP）作为酶组成的系统所示，该酶可以牢固地固定在这些材料中。 ALP不仅在这些材料中保持活性，而且基质还增强了酶的比活性，同时保护了它在高温下不失活。基质的存在可以通过改变接触溶液的盐浓度或温度来进行精细控制并显着提高反应速率。出色的可重复使用性，以及易于共固定其他有用成分（如磁性颗粒）的便利性，可轻松处理CoPEC，使这些材料成为用于固定小规模和大规模酶的酶固定支架的有趣候选材料。催化过程。

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  • 来源
    《Advanced Functional Materials》 |2013年第38期|4785-4792|共8页
  • 作者

    Patricia Tirado; Andreas Reisch; Emilie Roger; Fouzia Boulmedais; Loiec Jierry; Philippe Lavalle; Jean-Claude Voegel; Pierre Schaaf; Joseph B. Schlenoff; Benoit Frisch;

  • 作者单位

    Laboratoire de Conception et Application de Molecules Bioactives UMR 7199, CNRS/Universite de Strasbourg Faculte de Pharmacie 74 route du Rhin, 67401 Illkirch Cedex, France;

    Laboratoire de Conception et Application de Molecules Bioactives UMR 7199, CNRS/Universite de Strasbourg Faculte de Pharmacie 74 route du Rhin, 67401 Illkirch Cedex, France,CNRS UMR 7213, Laboratoire de Biophotonique et Pharmacologie, Universite de Strasbourg, Faculte de Pharmacie, 74 route du Rhin, F-67401 Illkirch CEDEX, France;

    Laboratoire de Conception et Application de Molecules Bioactives UMR 7199, CNRS/Universite de Strasbourg Faculte de Pharmacie 74 route du Rhin, 67401 Illkirch Cedex, France;

    Centre National de la Recherche Scientifique Institut Charles Sadron UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex, France;

    Centre National de la Recherche Scientifique Institut Charles Sadron UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex, France;

    Institut National de la Sante et de la Recherche Medicale INSERM Unite 1121, Universite de Strasbourg Faculte de Chirurgie Dentaire, 67000 Strasbourg, France;

    Institut National de la Sante et de la Recherche Medicale INSERM Unite 1121, Universite de Strasbourg Faculte de Chirurgie Dentaire, 67000 Strasbourg, France;

    Centre National de la Recherche Scientifique Institut Charles Sadron UPR 22, 23 rue du Loess, 67034 Strasbourg Cedex, France,Institut National de la Sante et de la Recherche Medicale INSERM Unite 1121, Universite de Strasbourg Faculte de Chirurgie Dentaire, 67000 Strasbourg, France,International Center for Frontier Research in Chemistry 67000 Strasbourg, France;

    Department of Chemistry and Biochemistry The Florida State University Tallahassee, FL 32306, USA;

    Laboratoire de Conception et Application de Molecules Bioactives UMR 7199, CNRS/Universite de Strasbourg Faculte de Pharmacie 74 route du Rhin, 67401 Illkirch Cedex, France,International Center for Frontier Research in Chemistry 67000 Strasbourg, France;

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