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首页> 外文期刊>Advanced Functional Materials >Screening Mesenchymal Stem Cell Attachment and Differentiation on Porous Silicon Gradients
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Screening Mesenchymal Stem Cell Attachment and Differentiation on Porous Silicon Gradients

机译：在多孔硅梯度上筛选间充质干细胞的附着和分化

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摘要

The profound effects that nanoscale surface topography exerts on celt behavior are highly relevant to the development of advanced biomaterials and to advances in tissue engineering and regenerative medicine. Here, an asymmetric anodization procedure is used to produce n-type porous silicon (pSi) gradients with pore sizes ranging from tens to hundreds of nanometers in diameter and changes in the ridge nanoroughness from a few to tens nanometers. Rat mesenchymal stem cells (rMSCs) adhere poorly at the regions with small pore size but high ridge roughness. Cell adhesion is increased gradually towards the large pore size but low ridge roughness end of the pSi gradients. Surface topography influences cell differentiation, but not cell proliferation. Osteogenesis of rMSCs is enhanced by porous topography with a ridge roughness lower than 10 nm, while adipogenesis of rMSCs is enhanced on the entire pSi gradient compared with flat Si substrates. The results demonstrate that the gradient format allows in-depth screening of surface parameters that are important for the control of mammalian cell behavior, thereby advancing the development of new and improved biomaterials for orthopaedic and tissue engineering applications.
机译：纳米级表面形貌对细胞行为的深远影响与先进生物材料的发展以及组织工程和再生医学的发展高度相关。在这里，使用不对称阳极氧化工艺来产生孔径范围从几十到几百纳米，脊纳米粗糙度从几纳米到几十纳米变化的n型多孔硅（pSi）梯度。大鼠间质干细胞（rMSCs）在孔径较小但脊粗糙度较高的区域粘附不良。朝向大孔径但pSi梯度的低脊粗糙度结束时，细胞粘附力逐渐增加。表面形貌影响细胞分化，但不影响细胞增殖。 rMSCs的成骨作用通过多孔形貌得以增强，脊的粗糙度低于10 nm，而与平坦的Si衬底相比，rMSCs的成脂作用在整个pSi梯度上得到了增强。结果表明，梯度格式允许深入筛选对于控制哺乳动物细胞行为很重要的表面参数，从而促进了整形外科和组织工程应用的新型和改良生物材料的开发。

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  • 来源
    《Advanced Functional Materials》 |2012年第16期|p.3414-3423|共10页
  • 作者

    Peng-Yuan Wang; Lauren R. Clements; Helmut Thissen; Andrew Jane; Wei-Bor Tsai; Nicolas H. Voelcker;

  • 作者单位

    Department of Chemical Engineering National Taiwan University No. 1, Roosevelt Rd., Sec. 4, Taipei, 106, Taiwan,School of Chemical and Physical Sciences Flinders University Bedford Park, SA 5042, Australia;

    School of Chemical and Physical Sciences Flinders University Bedford Park, SA 5042, Australia;

    CSIRO Materials Science and Engineering Bayview Avenue, Clayton VIC 3168, Australia;

    School of Chemical and Physical Sciences Flinders University Bedford Park, SA 5042, Australia;

    Department of Chemical Engineering National Taiwan University No. 1, Roosevelt Rd., Sec. 4, Taipei, 106, Taiwan;

    Mawson Institute University of South Australia Mawson Lakes, SA 5095, Australia;

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