• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta biomaterialia >Screening rat mesenchymal stem cell attachment and differentiation on surface chemistries using plasma polymer gradients
【24h】

Screening rat mesenchymal stem cell attachment and differentiation on surface chemistries using plasma polymer gradients

机译：使用血浆聚合物梯度筛选大鼠间充质干细胞在表面化学上的附着和分化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

It is well known that the surface chemistry of biomaterials is important for both initial cell attachment and the downstream cell response. Surface chemistry gradients are a new format that allows the screening of the subtleties of cell-surface interactions in high throughput. In this study, two surface chemical gradients were fabricated using diffusion control during plasma polymerization via a tilted mask. Acrylic acid (AA) plasma polymer gradients were coated on a uniform 1,7-octadiene (OD) plasma polymer layer to generate OD-AA plasma polymer gradients, whilst diethylene glycol dimethyl ether (DG) plasma polymer gradients were coated on a uniform AA plasma polymer layer to generate AA-DG plasma polymer gradients. Gradient surfaces were characterized by X-ray photoelectron spectroscopy, infrared microscopy mapping, profilometry, water contact angle (WCA) goniometry and atomic force microscopy. Cell attachment density and differentiation into osteo- and adipo-lineages of rat-bone-marrow mesenchymal stem cells (rBMSCs) was studied on gradients. Cell adhesion after 24 h culture was sensitive to the chemical gradients, resulting in a cell density gradient along the substrate. The slope of the cell density gradient changed between 24 and 6 days due to cell migration and growth. Induction of rBMSCs into osteoblast- and adipocyte-like cells on the two plasma polymer gradients suggested that osteogenic differentiation was sensitive to local cell density, but adipogenic differentiation was not. Using mixed induction medium (50% osteogenic and 50% adipogenic medium), thick AA plasma polymer coating (>40 nm thickness with similar to 11% COOH component and 35 degrees WCA) robustly supported osteogenic differentiation as determined by colony formation and calcium deposition. This study establishes a simple but powerful approach to the formation of plasma polymer based gradients, and demonstrates that MSC behavior can be influenced by small changes in surface chemistry. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
机译：众所周知，生物材料的表面化学对于初始细胞附着和下游细胞反应都很重要。表面化学梯度是一种新的格式，可以以高通量筛选细胞-表面相互作用的细微差别。在这项研究中，通过倾斜掩模在等离子体聚合过程中使用扩散控制制造了两个表面化学梯度。将丙烯酸（AA）血浆聚合物梯度涂在均匀的1,7-辛二烯（OD）血浆聚合物层上以产生OD-AA血浆聚合物梯度，而将二甘醇二甲醚（DG）血浆聚合物梯度涂在均匀AA上等离子体聚合物层以产生AA-DG等离子体聚合物梯度。梯度表面通过X射线光电子能谱，红外显微图，轮廓图，水接触角（WCA）测角法和原子力显微镜来表征。在梯度上研究了大鼠骨髓间充质干细胞（rBMSCs）的细胞附着密度以及向骨和脂肪细胞的分化。培养24小时后细胞粘附对化学梯度敏感，导致沿底物的细胞密度梯度。由于细胞迁移和生长，细胞密度梯度的斜率在24至6天之间变化。在两个血浆聚合物梯度上，将rBMSCs诱导为成骨细胞和脂肪细胞样细胞，提示成骨分化对局部细胞密度敏感，而成脂分化则不是。使用混合诱导培养基（50％成骨和50％成脂培养基），厚的AA等离子体聚合物涂层（> 40 nm厚度，具有相似的11％COOH成分和35度WCA），可通过集落形成和钙沉积确定强有力地支持成骨分化。这项研究建立了一种简单但有效的方法来形成基于等离子聚合物的梯度，并证明了MSC的行为可能受表面化学的微小变化影响。 （C）2014 Acta Materialia Inc.，由Elsevier Ltd.发行。保留所有权利。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号