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首页> 外文期刊>Advanced Functional Materials >Complexation-lnduced Biomimetic Long Range Fibrous Orientation in a Rigid-Flexible Block Copolymer Thermogel
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Complexation-lnduced Biomimetic Long Range Fibrous Orientation in a Rigid-Flexible Block Copolymer Thermogel

机译：刚性-柔性嵌段共聚物热凝胶中络合诱导的仿生长纤维取向

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摘要

The orientation of the organofibrous structure plays an important role not only in the biomineralization process but also in the extracellular matrix (ECM) of articular cartilage by providing cells with biomechanical cues. Here, it is reported that a long range nanofibrous orientation can be realized by a self-assembling ionic complex between (+)-charged amphiphilic peptide block copolymers with a rigid-flexible block structure (polyalanine-PLX-polyalanine; PA-PLX-PA) and (-)-charged hyaluronic acid (HA). A biomimetic 3D culture system encapsulating chondrocytes is formed by a temperature-sensitive sol-to-gel transition of the PA-PLX-PA/HA complex aqueous solution, which provides a compatible microenvironment for the cells. The cell proliferation and biomarker expression for articular cartilage are significantly improved in the PA-PLX-PA/HA complex system relative to the PA-PLX-PA or the commercially available Matrigel systems. In particular, noticeable cell clustering is observed in the PA-PLX-PA/HA complex system with the long range nanofibrous structure. This research suggests a new method for developing a nanofibrous structure using an amphiphilic peptide block copolymer and demonstrates its potential uses as a unique biomimetic cell-culture matrix.
机译：通过为细胞提供生物力学提示，有机纤维结构的取向不仅在生物矿化过程中而且在关节软骨的细胞外基质（ECM）中都起着重要作用。在这里，据报道，通过具有刚性-柔性嵌段结构的（+）-荷电两亲肽嵌段共聚物（聚丙氨酸-PLX-聚丙氨酸； PA-PLX-PA）之间的自组装离子络合物，可以实现长距离的纳米纤维取向。 ）和（-）带电的透明质酸（HA）。封装软骨细胞的仿生3D培养系统是通过PA-PLX-PA / HA复合物水溶液的温度敏感的溶胶-凝胶转变形成的，这为细胞提供了兼容的微环境。相对于PA-PLX-PA或市售的Matrigel系统，PA-PLX-PA / HA复合系统中关节软骨的细胞增殖和生物标志物表达得到显着改善。特别地，在具有长程纳米纤维结构的PA-PLX-PA / HA复合系统中观察到明显的细胞簇。这项研究提出了一种使用两亲性肽嵌段共聚物开发纳米纤维结构的新方法，并证明了其作为独特的仿生细胞培养基质的潜在用途。

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  • 来源
    《Advanced Functional Materials》 |2012年第24期|5118-5125|共8页
  • 作者

    Mm Hee Park; Bo Gyu Choi; Byeongmoon Jeong;

  • 作者单位

    Department of Chemistry and Nano Science Department of Bioinspired Science (WCU) Ewha Womans University Seoul, 120-750, Korea;

    Department of Chemistry and Nano Science Department of Bioinspired Science (WCU) Ewha Womans University Seoul, 120-750, Korea;

    Department of Chemistry and Nano Science Department of Bioinspired Science (WCU) Ewha Womans University Seoul, 120-750, Korea;

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