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首页> 外文期刊>Advanced Functional Materials >A Molecular Brush Approach to Enhance Quantum Yield and Suppress Nonspecific Interactions of Conjugated Polyelectrolyte for Targeted Far-Red/Near-Infrared Fluorescence Cell Imaging
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A Molecular Brush Approach to Enhance Quantum Yield and Suppress Nonspecific Interactions of Conjugated Polyelectrolyte for Targeted Far-Red/Near-Infrared Fluorescence Cell Imaging

机译：一种分子刷方法，可提高量子产率并抑制共聚电解质的非特异性相互作用，用于靶向远红/近红外荧光细胞成像

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摘要

A red-fluorescent conjugated polyelectrolyte (CPE, P2) is grafted with dense poly(ethylene glycol) (PEG) chains via click chemistry and subsequently modified with folic acid to form a molecular brush based cellular probe (P4). P4 self-assembles into a core-shell nanostructure in aqueous medium with an average size of 130 nm measured by laser light scattering. As compared to P2, P4 possesses not only a substantially higher quantum yield (11%), but also reduced nonspecific interactions with biomolecules in aqueous medium due to the shielding effect of PEC. In conjunction with its high photostability and low cytotoxicity, utilization of P4 as a far-redear-infrared cellular probe allows for effective visualization and discrimination of MCF-7 cancer cells from NIH-3T3 normal cells in a high contrast, selective, and nonviral manner. This study thus demonstrates a flexible molecular brush approach to overcome the intrinsic drawbacks of CPEs for advanced bioimaging applications.
机译：通过点击化学将红色荧光共轭聚电解质（CPE，P2）与致密的聚乙二醇（PEG）链接枝，然后用叶酸修饰以形成基于分子刷的细胞探针（P4）。 P4在水性介质中自组装成核-壳纳米结构，通过激光散射测量平均尺寸为130 nm。与P2相比，由于PEC的屏蔽作用，P4不仅具有高得多的量子产率（11％），而且还减少了与生物分子在水性介质中的非特异性相互作用。结合其高光稳定性和低细胞毒性，将P4用作远红/近红外细胞探针可以有效地可视化和区分MCF-7癌细胞与NIH-3T3正常细胞，并具有高对比度，选择性和选择性。非病毒方式。因此，这项研究证明了一种灵活的分子刷方法可以克服CPE在高级生物成像应用中的固有缺点。

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  • 来源
    《Advanced Functional Materials》 |2010年第17期|P.2770-2777|共8页
  • 作者

    Kan-Yi Pu; Kai Li; Bin Liu;

  • 作者单位

    Department of Chemical and Biomolecular Engineering National University of Singapore Singapore, 117576 (Singapore);

    rnDepartment of Chemical and Biomolecular Engineering National University of Singapore Singapore, 117576 (Singapore);

    rnDepartment of Chemical and Biomolecular Engineering National University of Singapore Singapore, 117576 (Singapore);

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