• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Advanced Functional Materials >Biofunctionalization Of Vertically Aligned Diamond Nanowires
【24h】

Biofunctionalization Of Vertically Aligned Diamond Nanowires

机译：垂直排列的金刚石纳米线的生物功能化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Vertically aligned diamond nanowires are biofunctionalized using aminophenyl linker molecules to bond nucleic acid molecules with a well-defined nanometer-sized spacing to the transducer. This novel DNA biosensor combines the outstanding electrochemical properties of diamond as a transducer with the controlled bonding of DNA molecules to the tips of nanowires by use of an electrochemical attachment scheme. Nucleic acid molecules are bonded in this way and dispersed to the transducer, giving rise to optimized hybridization kinetics of DNA. Negatively charged redox mediator molecules (Fe(CN)_6~(3-/4-)) are applied for DNA-hybridization sensing. Voltammetric detection of DNA hybridization by differential pulse voltammetry is performed with respect to its sensitivity and reproducibility. On a sensor area of 0.03 cm~2, a detection limit of 2.0 pM is achieved. As for the chemical stability of the DNA bonding to the diamond nanowires, no degradation over 30 hybridization/denaturation cycles could be detected. By use of this dilute DNA arrangement, single-base mismatch discrimination is achieved. Under the same conditions, smooth diamond modified with phenyl is not suitable for amperometric DNA sensing.
机译：使用氨基苯基接头分子将垂直排列的金刚石纳米线进行生物功能化，以将具有明确定义的纳米尺寸间距的核酸分子结合到换能器上。这种新型的DNA生物传感器结合了钻石作为换能器的出色电化学性能，并利用电化学附着方案将DNA分子与纳米线尖端的受控键结合在一起。核酸分子以这种方式键合并分散在传感器上，从而产生了优化的DNA杂交动力学。带负电的氧化还原介体分子（Fe（CN）_6〜（3- / 4-））用于DNA杂交传感。就其灵敏度和可重复性而言，通过差分脉冲伏安法对DNA杂交进行伏安检测。在0.03 cm〜2的传感器区域上，检测极限为2.0 pM。至于与金刚石纳米线键合的DNA的化学稳定性，在30个杂交/变性循环中未检测到降解。通过使用这种稀释的DNA排列，可以实现单碱基错配识别。在相同条件下，用苯基修饰的光滑钻石不适合用于安培DNA感测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号