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首页> 外文期刊>Advanced Functional Materials >One-Pot Preparation of Polymer-Enzyme-Metallic Nanoparticle Composite Films for High-Performance Biosensing of Glucose and Galactose
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One-Pot Preparation of Polymer-Enzyme-Metallic Nanoparticle Composite Films for High-Performance Biosensing of Glucose and Galactose

机译：一锅制备聚合物酶金属纳米粒子复合膜，用于葡萄糖和半乳糖的高性能生物传感

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摘要

New polymer-enzyme-metallic nanoparticle composite films with a high-load and a high-activity of immobilized enzymes and obvious electrocatalysis/ nano-enhancement effects for biosensing of glucose and galactose are designed and prepared by a one-pot chemical pre-synthesis/ electropolymerization (CPSE) protocol. Dopamine (DA) as a reductant and a monomer, glucose oxidase (GOx) or galactose oxidase (GaOx) as the enzyme, and HAuCl_4 or H_2PtCl_6 as an oxidant to trigger DA polymerization and the source of metallic nanoparticles, are mixed to yield polymeric bionanocomposites (PBNCs), which are then anchored on the electrode by electropolymerization of the remaining DA monomer. The prepared PBNC material has good biocompatibility, a highly uniform dispersion of the nanoparticles with a narrow size distribution, and high load/activity of the immobilized enzymes, as verified by transmission/scanning electron microscopy and electrochemical quartz crystal microbalance. The thus-prepared enzyme electrodes show a largely improved amperometric biosensing performance, e.g., a very high detection sensitivity (99 or 129 μA cm~(-2) mm~(-1) for glucose for Pt PBNCs on bare or platinized Au), a sub-micromolar limit of detection for glucose, and an excellent durability, in comparison with those based on conventional procedures. Also, the PBNC-based enzyme electrodes work well in the second-generation biosensing mode. The proposed one-pot CPSE protocol may be extended to the preparation of many other functionalized PBNCs for wide applications.
机译：通过一锅化学预合成/制备/制备了新型的高负载，高固定化酶活性，明显的电催化/纳米增强作用的生物聚合物-金属-金属纳米粒子复合膜，用于葡萄糖和半乳糖的生物传感。电聚合（CPSE）协议。将多巴胺（DA）作为还原剂和单体，将葡萄糖氧化酶（GOx）或半乳糖氧化酶（GaOx）作为酶，将HAuCl_4或H_2PtCl_6作为引发DA聚合的氧化剂和金属纳米颗粒的源，进行混合，以生成聚合的生物纳米复合材料（PBNC），然后通过剩余DA单体的电聚合将其锚定在电极上。制备的PBNC材料具有良好的生物相容性，纳米颗粒的高度均匀分散，窄尺寸分布以及固定化酶的高负载/活性，这已通过透射/扫描电子显微镜和电化学石英晶体微量天平证实。如此制备的酶电极显示出大大提高的安培生物传感性能，例如，非常高的检测灵敏度（对于裸露或镀铂Au上的Pt PBNC，葡萄糖的检测灵敏度为99或129μAcm〜（-2）mm〜（-1）），与基于常规程序的检测方法相比，检测葡萄糖的亚微摩尔限值和出色的耐久性。同样，基于PBNC的酶电极在第二代生物传感模式下也能很好地工作。提议的一锅式CPSE协议可以扩展到为广泛的应用准备许多其他功能化的PBNC。

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  • 来源
    《Advanced Functional Materials》 |2009年第11期|1784-1791|共8页
  • 作者

    Yingchun Fu; Penghao Li; Qingji Xie; XiahongXu; Lihong Lei; Chao Chen; Can Zou; Wenfang Deng; Shouzhuo Yao;

  • 作者单位

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

    State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University, Changsha, 410082 (P. R. China);

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

    Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China College of Chemistry and Chemical Engineering Hunan Normal University Changsha, 410081 (P. R. China);

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