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A Direct, Multiplex Biosensor Platform for Pathogen Detection Based on Cross-linked Polydiacetylene (PDA) Supramolecules

机译：基于交联聚二乙炔（PDA）超分子的病原体检测的直接，多重生物传感器平台

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摘要

This study focuses on the development of a multiplex pathogen-detection platform based on polydiacetylene (PDA) using a novel immobilization procedure. PDA liposome-based solid sensors have a critical drawback as the PDA liposomes are not stably immobilized onto the solid substrate. Therefore, to overcome this problem, an interlinker, ethylenediamine, is introduced, which acts as a cross-linker between individual PDA liposomes. The quantity of ethylenediamine added was optimized to 1 mm, as measured by the fluorescence signal emitted by the stably immobilized PDA liposomes, a concentration at which the fluorescence signal is 10 times higher than for the resulting PDA chips made without the interlinker. This procedure is used to manufacture PDA liposome-based multiplex biosensor arrays for well-known water and food-borne pathogens. The fabricated biosensor was able to perform the simultaneous and quantitative detection of 6 species of pathogens. As such, the results demonstrated from this research can be exploited for the development of more advanced PDA-based biosensors and diagnostics.
机译：这项研究的重点是使用新型固定程序开发基于聚二乙炔（PDA）的多重病原体检测平台。基于PDA脂质体的固体传感器具有严重的缺点，因为PDA脂质体无法稳定地固定在固体基质上。因此，为了克服这个问题，引入了一种互连剂乙二胺，其充当各个PDA脂质体之间的交联剂。通过稳定固定的PDA脂质体发出的荧光信号测得的乙二胺添加量最优化为1 mm，该浓度下荧光信号的浓度比不使用互连剂制得的PDA芯片高10倍。此过程用于制造基于PDA脂质体的多重生物传感器阵列，用于已知的水和食源性病原体。所制造的生物传感器能够同时定量检测6种病原体。这样，这项研究证明的结果可用于开发更先进的基于PDA的生物传感器和诊断程序。

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  • 来源
    《Advanced Functional Materials》 |2009年第23期|3703-3710|共8页
  • 作者

    Cheol Hee Park; Jun Pyo Kim; Sang Wook Lee; Noo Li Jeon; Pil J. Yoo; Sang Jun Sim;

  • 作者单位

    Nano-optics and Biomolecular Engineering National Laboratory School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea);

    Nano-optics and Biomolecular Engineering National Laboratory School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea);

    Nano-optics and Biomolecular Engineering National Laboratory School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea);

    Department of Biomedical Engineering University of California Irvine, CA, 92697 (USA);

    SKKU Advanced Institute of Nanotechnology (SAINT) and School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea);

    Nano-optics and Biomolecular Engineering National Laboratory School of Chemical Engineering Sungkyunkwan University Suwon 440-746 (Korea);

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