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Direct-Write Assembly of Microperiodic Silk Fibroin Scaffolds for Tissue Engineering Applications

机译：用于组织工程应用的微周期丝素蛋白支架的直接写入组装。

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摘要

Three-dimensional, microperiodic scaffolds of regenerated silk fibroin have been fabricated for tissue engineering by direct ink writing. The ink, which consisted of silk fibroin solution from the Bombyx mori silkworm, was deposited in a layer-by-layer fashion through a fine nozzle to produce a 3D array of silk fibers of diameter 5 μm. The extruded fibers crystallized when deposited into a methanol-rich reservoir, retaining a pore structure necessary for media transport. The rheological properties of the silk fibroin solutions were investigated and the crystallized silk fibers were characterized for structure and mechanical properties by infrared spectroscopy and nanoindentation, respectively. The scaffolds supported human bone marrow-derived mesenchymal stem cell (hMSC) adhesion, and growth. Cells cultured under chondrogenic conditions on these scaffolds supported enhanced chondrogenic differentiation based on increased glucosaminoglycan production compared to standard pellet culture. Our results suggest that 3D silk fibroin scaffolds may find potential application as tissue engineering constructs due to the precise control of their scaffold architecture and their biocompatibility.
机译：再生的丝素蛋白的三维微周期支架已经通过直接墨水书写用于组织工程。该油墨由来自Bombyx mori桑蚕的丝素蛋白溶液组成，通过细喷嘴逐层沉积，以产生直径5μm的3D丝纤维阵列。挤出的纤维沉积到富含甲醇的储层中时会结晶，保留了介质运输所必需的孔结构。研究了丝素蛋白溶液的流变性，并通过红外光谱和纳米压痕分别表征了结晶的丝纤维的结构和力学性能。支架支持人类骨髓源间充质干细胞（hMSC）粘附和生长。在软骨形成条件下在这些支架上培养的细胞与标准团粒培养相比，基于增加的葡糖胺聚糖生产，支持增强的软骨形成分化。我们的结果表明，由于3D丝素蛋白支架的精确控制和生物相容性，其可能在组织工程构造中得到潜在应用。

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