Using Wool Keratin as a Basic Resist Material to Fabricate Precise Protein Patterns

Zhu Shuihong; Zeng Wenbin; Meng Zhaohui; Luo Wenhao; Ma Liyun; Li Yanran; Lin Changxu; Huang Qiaoling; Lin Youhui; Liu Xiang Yang

首页> 外文期刊>Advanced Materials >Using Wool Keratin as a Basic Resist Material to Fabricate Precise Protein Patterns

【24h】

Using Wool Keratin as a Basic Resist Material to Fabricate Precise Protein Patterns

机译：使用羊毛角蛋白作为基本的抵抗材料来制作精确的蛋白质图案

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The ability to pattern natural polymers at different scales is extremely important for many research areas, such as cell culture, regenerative medicine, bioelectronics, tissue engineering, degradable implants, and photonics. For the first time, the use of wool keratin (WK) as a structural biomaterial for fabricating precise protein microarchitectures is presented. Through straightforward biochemical processes, modified WK proteins become intrinsically photoreactive without significant changes in protein structure or function. Under light irradiation, intermolecular chemical crosslinking between WK molecules can be successfully initiated by using commercially available photoinitiators. As a result, high-performance WK patterning on the micrometer scale (mu m) can be achieved through a combination of water-based photolithography techniques. By simply mixing with nanoparticles, enzymes, and other dopants, various "functional WK resists" can be generated. In addition, without the addition of any cell-adhesive ligands, these patterned protein microstructures are demonstrated as bio-friendly cellular substrates for the spatial guidance of cells on their surface. Furthermore, periodic microfabricated WK structures in complex patterns that display typical iridescent behavior can be designed and formed over macroscale areas (cm).

机译：在许多研究领域，例如细胞培养，再生医学，生物电子学，组织工程，可降解植入物和光子学，对不同规模的天然聚合物进行构图的能力极为重要。首次提出了使用羊毛角蛋白（WK）作为结构生物材料来制造精确的蛋白质微体系结构。通过简单的生化过程，修饰的WK蛋白本质上具有光反应性，而蛋白质结构或功能没有明显变化。在光照射下，可以通过使用市售的光引发剂成功地引发WK分子之间的分子间化学交联。结果，可以通过结合水基光刻技术来实现微米级（μm）的高性能WK图案化。通过简单地与纳米颗粒，酶和其他掺杂剂混合，可以产生各种“功能性WK抗蚀剂”。另外，在不添加任何细胞粘附配体的情况下，这些图案化的蛋白质微结构被证明是生物友好的细胞底物，用于细胞在其表面上的空间引导。此外，可以设计并在宏观区域（cm）上形成具有典型虹彩行为的复杂图案的周期性微加工WK结构。

著录项

来源
《Advanced Materials》 |2019年第28期|1900870.1-1900870.7|共7页
作者
Zhu Shuihong; Zeng Wenbin; Meng Zhaohui; Luo Wenhao; Ma Liyun; Li Yanran; Lin Changxu; Huang Qiaoling; Lin Youhui; Liu Xiang Yang;
展开▼
作者单位

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China;

Xiamen Univ, Res Inst Biomimet & Soft Matter, Dept Phys, Fujian Prov Key Lab Soft Funct Mat Res,Jiujiang R, Xiamen 361005, Fujian, Peoples R China|Natl Univ Singapore, Dept Phys, FOS, 2 Sci Dr 3, Singapore 117542, Singapore;

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类
关键词
biomaterials; hierarchical materials; photolithography; protein microarchitectures; wool keratin;

机译：生物材料;分层材料;光刻;蛋白质微体系结构;羊毛角蛋白;

相似文献

外文文献
中文文献
专利

1. Using Wool Keratin as a Basic Resist Material to Fabricate Precise Protein Patterns [J] . Zhu Shuihong, Zeng Wenbin, Meng Zhaohui, Advanced Materials . 2019,第28期

机译：使用羊毛角蛋白作为碱性抗蚀剂以制造精确的蛋白质图案
2. Expression patterns of keratin intermediate filament and keratin associated protein genes in wool follicles. [J] . Yu Z, Gordon SW, Nixon AJ, Differentiation: The Journal of the International Society of Differentiation . 2009,第3期

机译：毛囊中角蛋白中间丝和角蛋白相关蛋白基因的表达模式。
3. Biopatterning: Precise Protein Photolithography (P 3 ): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist (Adv. Sci. 9/2017) [J] . Ying Mao, Yuanzhe Yang, Guozheng Zhang, Advanced Science . 2017,第9期

机译：生物图案：精确的蛋白质光刻（P 3）：使用丝素蛋白轻链作为抗性的高性能生物图案（Adv。Sci。9/2017）
4. Preliminary linkage studies in sheep of keratin and keratin-associated protein genes with fleece weight, wool fibre diameter and fibre curvature [C] . SH Phua, DR Scobie, D 0Connell, Annual Conference of the New Zealand Society of Animal Production . 2015

机译：角蛋白和角蛋白相关蛋白基因绵羊绵羊的初步联系研究，羊毛纤维直径和纤维曲率
5. Keratin from Human Hair and Sheep Wool: Characterisation and Its Uses in the Fabrication of Hydrogels [D] . Mondragon De La Cruz, David. 2020

机译：来自人头头发和绵羊羊毛的角蛋白：特征及其在水凝胶制造中的用途
6. Biopatterning: Precise Protein Photolithography (P3): High Performance Biopatterning Using Silk Fibroin Light Chain as the Resist (Adv. Sci. 9/2017) [O] . Wanpeng Liu, Zhitao Zhou, Shaoqing Zhang, 2017

机译：生物图案：精确的蛋白质光刻（P3）：使用丝素蛋白轻链作为抗性的高性能生物图案（Adv。Sci。9/2017）
7. pH-sensitive resist materials for combined photolithographic patterning of proteins and fluid lipid bilayers [O] . Shah Mirat 2008

机译：pH敏感的抗蚀剂材料，用于蛋白质和流体脂质双层的组合光刻图案化

Using Wool Keratin as a Basic Resist Material to Fabricate Precise Protein Patterns

摘要

著录项

相似文献

相关主题

期刊订阅