Straining For Better DNA Probes

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Straining For Better DNA Probes

机译：应变以获得更好的DNA探针

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DNA arrays have become standard tools in genomic research, as they allow the parallel measurement of relative gene expression levels across different samples and with minimal sample use. An important measure of their effectiveness is the number of DNA probes per surface area; more information can be extracted per experiment as the probe density isrnincreased. A new fabrication technique for such arrays has now been developed by a group of Canadian researchers. Their technique, based on soft lithography, relies on the use of an elastomeric stamp, which is stretched uniformly in two dimensions to maximize its surface area. A DNA array is then printed on the stamp using conventional methods, and the strain is removed, resulting in an array probe density up to an order of magnitude greater than previous constructions.

机译：DNA阵列已成为基因组研究的标准工具，因为它们可以并行测量不同样品之间的相对基因表达水平，并且样品使用量最少。衡量其有效性的一个重要指标是每个表面积的DNA探针数量。随着探针密度的增加，每个实验都可以提取更多信息。一组加拿大研究人员现已开发出一种用于此类阵列的新制造技术。他们的技术基于软光刻技术，依靠使用弹性体印模，该印模在二维方向上均匀拉伸以最大化其表面积。然后使用常规方法将DNA阵列印在印章上，并去除应变，从而使阵列探针的密度比以前的结构高出一个数量级。

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《Advanced Materials》 |2009年第29期|a5-a5|共1页
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1. DNA Probes Show Genetic Variation in Cyanobacterial Symbionts of the Azolla Fern and a Closer Relationship to Free-Living Nostoc Strains than to Free-Living Anabaena Strains [J] . Jacek Plazinski, Qi Zheng, Rona Taylor, Applied and Environmental Microbiology . 1990,第5期

机译：DNA探针显示满江红的蓝藻共生体中的遗传变异，与自由生活的念珠菌菌株的关系比与自由生活的鱼腥藻菌株的亲缘关系更紧密。
2. Detection of mutations in the rpoB gene of rifampicin-resistant Mycobacterium tuberculosis strains inhibiting wild type probe hybridization in the MTBDR plus assay by DNA sequencing directly from clinical specimens [J] . Sinha Pallavi, Srivastava G. N., Tripathi Rajneesh, BMC Microbiology . 2020,第a期

机译：通过直接来自临床标本，通过直接从临床标本进行DNA测序抑制MTBDR Plus测定中的利福平抗性分枝杆菌结核分枝杆菌菌株的RPOB基因突变
3. Synthesis and Assessment of DNA/Silver Nanoclusters Probes for Optimal and Selective Detection of Tristeza Virus Mild Strains [J] . Shokri Ehsan, Hosseini Morteza, Faridbod Farnoush, Journal of Fluorescence . 2016,第5期

机译：DNA /银纳米簇探针的合成和评估，用于特氏青霉病毒轻菌株的最佳和选择性检测。
4. Microcosm Assessment of a DNA-Probe Applied to Aerobic Degradation of cis-1,2-Dichloroethene by Polaromonas sp. Strain JS666 [C] . Cloelle Giddings, Laura Jennings, James M. Gossett International Conference on Remediation of Chlorinated adn Recalcitrant Compounds . 2010

机译：通过极化菌族评估DNA探针的DNA探针通过极化肌肉菌均采用CIS-1,2-二氯乙烯的有氧降解。菌株JS666.
5. Mechanism of electron transfer in double-stranded DNA and PNA-DNA hybrids, and the development of a fluorescence probe for DNA and RNA detection. [D] . Ly, Danith. 1998

机译：电子在双链DNA和PNA-DNA杂化物中转移的机理，以及用于DNA和RNA检测的荧光探针的发展。
6. DNA Probes Show Genetic Variation in Cyanobacterial Symbionts of the Azolla Fern and a Closer Relationship to Free-Living Nostoc Strains than to Free-Living Anabaena Strains [O] . Jacek Plazinski, Qi Zheng, Rona Taylor, 1990

机译：DNA探针显示在满江红蓝藻共生细菌中的遗传变异与自由生活的念珠菌菌株的关系比与自由生活的鱼腥藻菌株的亲缘关系更紧密。
7. Filamentous Chloroflexi (green non-sulfur bacteria) are abundant in wastewater treatment processes with biological nutrient removal c cThe EMBL accession numbers for the sequences reported in this paper are X84472 (strain SBR1029 16S rDNA), X84474 (strain SBR1031 16S rDNA), X84498 (strain SBR1064 16S rDNA), X84565 (strain SBR2022 16S rDNA), X84576 (strain SBR2037 16S rDNA) and X84607 (strain SBR2076 16S rDNA). [O] . Lovisa Björnsson, Philip Hugenholtz, Gene W. Tyson, 2002

机译：丝状氯克莱克莱（绿色非硫磺细菌）在废水处理过程中具有生物营养处理方法，本文报道的序列的EMBL登录号是X84472（菌株SBR1029 16S RDNA），X84474（菌株SBR1031 16S RDNA），X84498（菌株SBR1064 16S rDNA），X84565（菌株SBR2022 16S rDNA），X84576（菌株SBR2037 16S rDNA）和X84607（菌株SBR2076 16S RDNA）。

Straining For Better DNA Probes

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