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首页> 外文期刊>Advanced Materials >Surface-Enhanced Raman Detection on Metalized Nanostructured Poly(p-xylylene) Films
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Surface-Enhanced Raman Detection on Metalized Nanostructured Poly(p-xylylene) Films

机译：金属化的纳米结构聚对二甲苯薄膜的表面增强拉曼检测

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摘要

Surface-enhanced Raman spectroscopy (SERS) as the basis of rapid and reliable biosensing techniques has the significant advantages of minimal sample preparation and ease of operation, when compared with other methods. The SERS technique has received much attention over the years owing to its ability to reach single-molecule detection and for applications such as rapid DNA sequencing. Its use for biomedical analysis, however, has lagged behind because of the difficulties in finding effective substrates for accommodating biological entities that also provide high sensitivity and highly reproducible SERS responses from sample to sample. If these challenges are met, SERS could potentially be extremely useful in the field of medical diagnostics to detect infectious agents. Here, we report the development of SERS substrates that provide a highly uniform and reproducible bioanalysis surface and demonstrate examples of "fingerprint" signals from different types of bacteria, in which the signals differ based on variations in the cell membranes. Our method is unique in controlling surface morphology and topology prior to SERS-active metal deposition. As a result, we created a novel substrate that has a uniform and reproducible SERS signal over a large ( > 1 mm~2 ) surface. The advantage of using these new types of substrates is that no template or lithography is involved, thus providing a simple, inexpensive, and quick method to achieve highly sensitive and spatially uniform SERS signals for biomedical applications.
机译：与其他方法相比，作为快速可靠的生物传感技术的基础的表面增强拉曼光谱（SERS）具有最小的样品制备和易于操作的显着优势。多年来，SERS技术由于能够实现单分子检测以及用于快速DNA测序等应用而备受关注。但是，由于难以找到有效的底物来容纳生物实体，而生物实体也提供了高灵敏度和高重现性的SERS响应，因此其在生物医学分析中的应用一直滞后。如果能够解决这些挑战，SERS在医学诊断领域以检测传染原可能会非常有用。在这里，我们报告了SERS底物的发展，该底物可提供高度均匀且可重现的生物分析表面，并展示了来自不同类型细菌的“指纹”信号示例，其中信号根据细胞膜的变化而不同。在SERS活性金属沉积之前，我们的方法在控制表面形态和拓扑方面是独特的。结果，我们创建了一种新颖的基板，该基板在较大（> 1 mm〜2）的表面上具有均匀且可再现的SERS信号。使用这些新型基板的优势在于不涉及模板或光刻技术，从而提供了一种简单，廉价且快速的方法来实现用于生物医学应用的高度敏感且空间均匀的SERS信号。

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