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首页> 外文期刊>Aerobiologia >Detection of immunogens from Fraxinus spp. pollen grains
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Detection of immunogens from Fraxinus spp. pollen grains

机译：从水曲柳属物种检测免疫原。花粉粒

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摘要

This work is aimed at characterising immunogens from Fraxinus uhdei pollen and to develop ELISA assays for their immunodetection, since it is a relevant aeroallergen. F. uhdei pollen extract contained proteins in a wide range of molecular mass from 22 to 112 kDa by SDS-PAGE; moreover, 187 fractions were detected in the 2D electrophoresis. Pollen extract was used to generate rabbit hyperimmune serum. Western blot revealed six main immunogens in a range from 40 to 21 kDa and several protein spots in 2D immunoblot. Tryptic peptides from six spots were analysed by nano-LC-ESI-MS/MS, indicating that most of proteins show homology with proteins involved in metabolism and with Ole e 11 and Fra e 2. The minimum concentration of Fraxinus spp. proteins detected by ELISA assays was 0.25 mu g/mL. The detection of one 21.5 KDa protein by WB in the dust obtained from air sampling suggests the possibility to be a Fraxinus spp. protein suspended in the atmosphere. ELISA assays of the air samples detected the higher concentrations of Fraxinus spp. proteins when its airborne pollen concentrations were higher, according to the Mexican Aerobiology Network.
机译：这项工作旨在表征水曲柳花粉的免疫原，并开发用于其免疫检测的ELISA测定法，因为它是一种相关的气敏原。通过SDS-PAGE，F。uhdei花粉提取物包含分子量范围从22至112 kDa的蛋白质。此外，在2D电泳中检测到187个馏分。花粉提取物用于产生兔超免疫血清。 Western印迹揭示了6种主要的免疫原，范围从40到21 kDa，并在2D免疫印迹中有几个蛋白斑点。通过nano-LC-ESI-MS / MS分析了六个斑点中的胰蛋白酶肽，表明大多数蛋白质与代谢相关蛋白质以及Ole e 11和Fra e 2具有同源性。Fraxinus spp的最低浓度。通过ELISA测定检测到的蛋白质为0.25μg/ mL。 WB在从空气采样获得的粉尘中检测到一种21.5 KDa的蛋白质，提示可能是水曲霉。蛋白质悬浮在大气中。空气样品的ELISA分析检测到较高的水曲霉菌种浓度。根据墨西哥航空生物学网的报道，当空气中的花粉浓度较高时，蛋白质就会被释放。

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  • 来源
    《Aerobiologia》 |2015年第3期|403-410|共8页
  • 作者

    Robledo-Retana Tania; Zenteno E.; Agundis-Mata M. C.; Pereyra-Morales M. A.; Calderon-Segura M. E.; Calderon-Ezquerro M. C.;

  • 作者单位

    Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Med, Mexico City 04510, DF, Mexico|Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Mexico City 04510, DF, Mexico;

    Asociac Evitar Ceguera IAP Hosp Dr Luis Sanchez B, Coyoacan 04030, DF, Mexico|Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Med, Mexico City 04510, DF, Mexico;

    Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Med, Mexico City 04510, DF, Mexico;

    Univ Nacl Autonoma Mexico, Dept Bioquim, Fac Med, Mexico City 04510, DF, Mexico;

    Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Mexico City 04510, DF, Mexico;

    Univ Nacl Autonoma Mexico, Ctr Ciencias Atmosfera, Mexico City 04510, DF, Mexico;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Aeroallergens; ELISA; Fraxinus; Immunoproteomics;

    机译：气变应原;酶联免疫吸附;水曲柳;免疫组学;

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