...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Aerobiologia >Effect of environmental pollution on the proteins, allergenic bands, ontogeny and structure of Avicennia marina (Forsk.) Vierh (Avicenniaceae) pollen grains
【24h】

Effect of environmental pollution on the proteins, allergenic bands, ontogeny and structure of Avicennia marina (Forsk.) Vierh (Avicenniaceae) pollen grains

机译：环境污染对Avicennia marina（Forsk。）Vierh（Avicenniaceae）花粉粒的蛋白质，过敏原带，个体发育和结构的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In Bushehr province of Iran, Avicennia marina trees have grown in Bordekhoon (Mond Protected Area) and Assaluyeh (Marine National Park of Nayband). Contrary to Bordekhoon, Assaluyeh is a petrochemical region with environmental pollution. This study was aimed to studying protein profiles, allergenic bands, ontogeny, structure and elemental composition of tectum of A. marina pollens in Assaluyeh and Bordekhoon. Pollens were collected from two regions and extracted in PBS, and protein profiles of pollens were determined by sulfate-poly-acrylamide gel electrophoresis (SDS-PAGE). As an experimental model, 20 female 6-8-week-old Balb/C mice were divided into two groups. The mice of first and second groups were sensitized by Bordekhoon and Assaluyeh pollen extracts, respectively, and mice serum samples were used for immunoblotting. Pollen characteristics were studied using light and scanning electron microscopes. SDS-PAGE showed some differences between pollen protein profiles of two regions. Immunoblotting assay detected that pollens have two allergenic bands and the protein band at 100 KD is the common allergenic protein in two regions. Light microscopy revealed that the development of anther wall was basic type and some abnormalities were observed in microspores and pollens of Assaluyeh. Scanning electron microscopy studies showed that the apertures in considerable numbers of Assaluyeh pollens were closed. The comparison of elemental composition of pollen tectum between two regions showed that pollens of Assaluyeh have accumulated Cu on their tectum. Results obtained indicated that environmental pollution can affect protein profile, allergenic bands, structure and elemental composition of tectum of A. marina pollens.
机译：在伊朗布什尔省，Avicennia滨海树种生长在Bordekhoon（蒙德保护区）和Assaluyeh（内班德国家海洋公园）上。与Bordekhoon相反，Assaluyeh是一个石化地区，具有环境污染。这项研究的目的是研究阿萨鲁耶和博德霍恩滨海花粉的蛋白质谱，过敏原带，个体发育，结构和元素组成。从两个区域收集花粉并在PBS中提取，并通过硫酸盐-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定花粉的蛋白质谱。作为实验模型，将20只6-8周大的Balb / C雌性小鼠分为两组。第一组和第二组的小鼠分别用Bordekhoon和Assaluyeh花粉提取物致敏，并将小鼠血清样品用于免疫印迹。使用光和扫描电子显微镜研究花粉特性。 SDS-PAGE显示两个区域的花粉蛋白谱之间存在一些差异。免疫印迹法检测到花粉具有两个过敏原带，而100 KD处的蛋白带是两个区域中常见的过敏原蛋白。光学显微镜检查显示，花药壁的发育是基本类型，并且在Assaluyeh的小孢子和花粉中观察到一些异常。扫描电子显微镜研究表明，相当数量的Assaluyeh花粉中的孔是封闭的。比较两个地区花粉支架的元素组成可知，Assaluyeh的花粉在其支架上积累了铜。结果表明，环境污染可以影响滨海花粉的蛋白质谱，过敏原带，结构和元素组成。

著录项

  • 来源
    《Aerobiologia》 |2014年第1期|59-69|共11页
  • 作者

    Mandana Salehi; Ahmad Majd; Parissa Jonoubi; Leila Karami; Gholamali Kardar; Zahra Pourpak;

  • 作者单位

    Faculty of Biological Sciences, Kharazmi University, Tehran, Iran;

    Department of Biology, Faculty of Biological Sciences, Is1amic Azad University, North Tehran Branch, Tehran, Iran;

    Faculty of Biological Sciences, Kharazmi University, Tehran, Iran;

    Department of Horticulture, Faculty of Agriculture, Persian Gulf University, Bushehr, Iran;

    Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran;

    Immunology, Asthma and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Environmental pollution; Avicennia marina; Immunoblot assay; Pollen ontogeny; Pollen tectum; SDS-PAGE;

    机译：环境污染;Avicennia码头;免疫印迹分析;花粉个体发育;花粉的顶盖;SDS页面;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号