摘要:Objective To establish a method for the determination of notoginsenoside R1and ginsenoside Rg1, Re, Rg2, Rb1and Rd in Panax Notoginseng health food by high performance liquid chromatography. Methods The analysis was carried out on an analytical column Kromasil 100-5-C18(4.6 mm×250 mm, 5 μm) with the mobile phase consisted of acetonitrile (A)-water (B) in gradient mode (0~25 min, 17%A→19%A; 25~45 min, 19%A→29%A;45~55 min, 29%A; 55~95 min, 29%A→40%A; 95~97 min, 40%A; 97~100 min, 40%A→17%A) at the detection wavelength of 203 nm and a flow rate of 1.0 mL/min. The column temperature was 40 . ℃ Results Notoginsenoside R1, ginsenoside Rg1, Re, Rg2, Rb1and Rd had good linear relationships in the range of 4.060~203.0, 4.818~240.9, 4.154~207.7, 4.282~214.1, 3.894~194.7, 4.124~206.2 μg/mL, respectively, and the correlation coefficients were more than 0.9985, the average recoveries (n=9) were more than 93.4% and RSDs were less than 2.5%. Conclution This method is accurate, highly sensitive and reproduciable, which can be used to control the quality of Panax Notoginseng health food.%目的 建立高效液相色谱法(high performance liquid chromatography, HPLC)测定三七类保健食品中三七皂苷R1、人参皂苷Rg1、人参皂苷Rg2、人参皂苷Rb1、人参皂苷Rd和人参皂苷Re含量的分析方法.方法 用Kromasil 100-5-C18(4.6 mm×250 mm, 5 μm)色谱柱, 以乙腈(A)-水(B)为流动相, 梯度洗脱(0~25 min, 17%A→19%A; 25~45 min, 19%A→29%A; 45~55 min, 29%A; 55~95 min, 29%A→40%A; 95~97 min, 40%A;97~100 min, 40%A→17%A), 流速1.0 mL/min, 检测波长203 nm, 柱温40 ℃.结果 三七皂苷R1、人参皂苷Rg1、Re、Rg2、Rb1、Rd分别在4.060~203.0, 4.818~240.9, 4.154~207.7, 4.282~214.1, 3.894~194.7, 4.124~206.2 μg/mL浓度范围内线性关系良好, 相关系数都在0.9985以上.该方法6种皂苷平均回收率均高于93.4%, 相对标准偏差(relative standard deviation, RSD)小于2.5%.结论 本法准确可靠、灵敏度高、重现性好, 可作为含三七类保健食品的质量控制方法.