首页> 外文期刊>Aerobiologia >Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles
【24h】

Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles

机译:暴露于气态污染物和车辆尾气颗粒后的梧桐花粉的蛋白质表达特征

获取原文
获取原文并翻译 | 示例
           

摘要

Being major ornamental street trees, species of Platanus are widely planted in the Shanghai urban area. A great deal of allergenic Platanus pollen is released from the trees and suspended in the atmosphere during its flowering season, ultimately causing allergic respiratory diseases. Few papers have focused on the distribution of this type of pollen and its expression of allergenic proteins. In order to investigate any differences in protein expression in Platanus pollen following exposure to gaseous and paniculate pollutants, a special apparatus was designed. Exposure condition (such as temperature, humidity, and exposure time) of Platanus pollen and gaseous pollutants can be simulated using of this apparatus. Fresh Platanus orientalis pollen, pollutant gases (NO_2, SO_2, NH_3), and typical urban ambient particles (vehicle exhaust particles, VEPs) were mixed in this device to examine possible changes that might occur in ambient airborne urban pollen following exposure to such pollutants. Our results showed that the fresh P. orientalis pollen became swollen, and new kinds of particles could be found on the surface of the pollen grains after exposure to the pollutants. The results of SDS-PAGE showed that five protein bands with molecular weights of 17-19, 34, 61, 82, and 144 kDa, respectively, were detected and gray scale of these brands increased after the pollen exposure to gaseous pollutants. The two-dimensional gel electro-phoresis analysis demonstrated that a Platanus pollen allergenic protein (Pla a1, with a molecular weight of 18 kDa) increased in abundance following exposure to pollutant gases and VEPs, implying that air pollutants may exacerbate the allergenicity of pollen.
机译:作为主要的观赏街道树木,法国梧桐在上海市区广泛种植。大量的过敏性桔梗花粉在开花期从树木中释放出来并悬浮在大气中,最终导致过敏性呼吸系统疾病。很少有论文关注这种花粉的分布及其致敏蛋白的表达。为了研究暴露于气态和颗粒污染物后的法国梧桐花粉中蛋白质表达的任何差异,设计了一种特殊的仪器。可以使用该设备模拟梧桐花粉和气态污染物的暴露条件(例如温度,湿度和暴露时间)。将新鲜的东方侧柏花粉,污染物气体(NO_2,SO_2,NH_3)和典型的城市环境颗粒(车辆排气颗粒,VEP)混合在此设备中,以检查暴露于此类污染物后环境空气传播的城市花粉中可能发生的变化。我们的结果表明,新鲜的东方侧柏花粉变得膨胀,并且在暴露于污染物之后,在花粉颗粒的表面上会发现新的颗粒。 SDS-PAGE结果显示,在花粉暴露于气态污染物后,分别检测到5个分子量分别为17-19、34、61、82和144 kDa的蛋白带,这些品牌的灰度级增加。二维凝胶电泳分析表明,暴露于污染气体和VEPs后,法国梧桐花粉致敏蛋白(分子量为18 kDa的Pla a1)的含量增加,这表明空气污染物可能会加剧花粉的致敏性。

著录项

  • 来源
    《Aerobiologia》 |2014年第3期|281-291|共11页
  • 作者单位

    School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;

    School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;

    School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;

    School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;

    School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;

    School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;

    School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;

    Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China;

    Center for Environmental Science in Saitama, Saitama 374-0115, Japan;

    School of Science and Engineering, Saitama University, Saitama 338-8570, Japan;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Platanus pollen; Air pollution; Protein expression;

    机译:梧桐花粉空气污染;蛋白表达;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号