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首页> 外文期刊>Aerobiologia >Recovery of germinating fungal conidia from the nasal cavity after environmental exposure
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Recovery of germinating fungal conidia from the nasal cavity after environmental exposure

机译：环境暴露后从鼻腔中回收萌发的真菌分生孢子

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Background The expression of fungal allergens is increased by the germination of conidia. We assessed the state of germination of fungal conidia recovered by nasal lavage after environmental exposure. Methods Nasal lavage was performed on twenty adults at three stages: the start of the experiment, after 1 h indoors, and after 1 h outdoors. One half of the lavage liquid was immediately treated to prevent in-vitro germination and stained with periodic acid Schiff (PAS) to enable identification of germinated and ungerminated conidia. The untreated half of the lavage liquid was cultured on nutrient agar plates to enumerate and identify viable fungi. Results PAS staining showed that both ungerminated and germinated conidia, and hyphal fragments, were present in the nasal cavity. The most prevalent fungi recovered were Aspergillus, Alternaria, Cladosporium, Epicoccum, Penicil-lium, and Yeast species. The number of viable fungi recovered after 1 h indoors was significantly less than after 1 h outdoors (P < 0.01). Conclusions Viable fungi and germinating conidia, in addition to ungerminated conidia and hyphal fragments, were present in the nasal cavity after both indoor and outdoor exposure. This provides novel insight into the pathogenicity of exposure to fungal aeroallergens.
机译：背景通过分生孢子的萌发增加了真菌过敏原的表达。我们评估了环境暴露后通过鼻灌洗恢复的真菌分生孢子的萌发状态。方法对20名成年人进行三个阶段的洗鼻：实验开始，室内1 h和室外1 h。立即处理一半的灌洗液以防止体外萌发，并用高碘酸席夫（PAS）染色，以鉴定萌发和未萌发的分生孢子。未经处理的一半灌洗液在营养琼脂平板上培养，以枚举并鉴定有活力的真菌。结果PAS染色显示鼻腔中同时存在未发芽和发芽的分生孢子和菌丝碎片。回收的最普遍的真菌是曲霉菌，链格孢菌，枝孢菌，埃克球菌，青霉属和酵母菌。室内1 h后回收的活菌数量明显少于室外1 h后的回收率（P <0.01）。结论室内和室外暴露后，鼻腔中还存在活菌和萌发分生孢子，以及未萌发的分生孢子和菌丝碎片。这为暴露于真菌气变应原的致病性提供了新颖的见解。

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