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Detection of aerosolized bacterial spores (Bacillus atrophaeus) using free-flying honey bees (Hymenoptera: Apidae) as collectors

机译:使用自由飞行的蜜蜂(膜翅目:Apidae)作为收集器来检测雾化的细菌孢子(Atrophaeus)

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摘要

An aerosol cloud of Bacillus atrophaeus (previously B. subtilis variety niger) spores, an anthrax surrogate, was created in a large 0.4 ha (1 ac), bee-containing, open-mesh tent. Bees from a B. atrophaeus uncon-taminated hive flying through the cloud adsorbed the spores in statistically significant quantities. After removal of the B. atrophaeus contaminated hive and introduction of another B. atrophaeus uncontaminated hive, the bees again were monitored for the next few days for B. atrophaeus spores. B. atrophaeus spores accumulated on the bees' bodies following their exposure to the residual B. atrophaeus contamination in the tent. The spore loads on the bees quickly returned to background levels after the hives were removed from the contaminated tent area. It may therefore be practical to use honey bee colonies to monitor foraging areas for disease-causing spores.
机译:在一个大的0.4公顷(1 ac),含蜂的开放式网状帐篷中,形成了一种萎缩芽孢杆菌(以前是枯草芽孢杆菌变种Niger)孢子的气溶胶云,一种炭疽替代物。未被污染的密闭芽孢杆菌的蜂巢飞过云层,在统计上显着地吸收了孢子。在去除了受到B. atrophaeus污染的蜂巢并引入了另一种未经B. aphaphaeus污染的蜂巢之后,在接下来的几天中再次监测蜜蜂的B. atrophaeus孢子。暴露于帐篷中残留的B. atrophaeus污染后,B。atrophaeus孢子在蜜蜂身上积聚。将蜂箱从受污染的帐篷区域移走后,蜜蜂的孢子负荷迅速恢复到本底水平。因此,使用蜜蜂群落来监测致病孢子的觅食区域可能是实用的。

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