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首页> 外文期刊>Aerobiologia >Temporal patterns of airborne Phytophthora spp. in a woody plant nursery area detected using real-time PCR
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Temporal patterns of airborne Phytophthora spp. in a woody plant nursery area detected using real-time PCR

机译：空气传播的植物植物的时间模式。在使用实时PCR检测到的木质植物苗圃中

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摘要

In this study, spore trap monitoring was applied to provide a proof of concept for the use of qPCR to detect Phytophthora in aerial samples and provide valuable information for epidemiological studies in nurseries. Two qPCR TaqMan assays were developed to detect pathogen DNA: the first used a generic probe to detect Phytophthora spp., and the second was based on a specific probe for detecting P. ramorum and P. lateralis. All samples tested positive for the genus Phytophthora, although P. ramorum and P. lateralis were not detected. In late spring and in autumn, two main peaks of Phytophthora sporulation were observed. Peaks were preceded by rainfall, high relative humidity, and mild temperature. From mid-May to the end of August, Phytophthora DNA detected in the air increased with relative humidity, while it decreased with increasing mean temperature. There was also a positive correlation between Phytophthora DNA detected and rainfall in the same period. No significant correlations between Phytophthora DNA and temperature or rainfall were found from the end of August to December. Our results are in agreement with those obtained with classical diagnostic methods based on microscopy, but the approach used here enabled rapid detection and relative quantification of the target organisms, thus assisting in the implementation of disease management strategies.
机译：在这项研究中，孢子陷阱监测被应用于使用QPCR在空中样品中检测植物对植物样本中的植物酞植物的概念证据，并为托儿所提供有价值的流行病学信息。开发出两种QPCR Taqman测定以检测病原体DNA：首先使用通用探针来检测植物胰酞菁SPP。，第二个是基于用于检测P. Ramorum和P. Lastalis的特定探针。虽然未检测到P. Ramorum和P. Lasterlis，但所有样品都测试了植物属的阳性。在春季和秋季，观察了两种植物孢子孢子素的两个主要峰。山峰以降雨，高相对湿度和温度温度。从5月中旬到8月底，在空气中检测到的植物Thora DNA随着相对湿度而增加，同时随着平均温度的增加而降低。在同一时期检测到植物炎季洛拉DNA与降雨之间也存在正相关性。从8月底到12月，发现了植物Thora DNA和温度或降雨之间没有显着相关性。我们的结果与基于显微镜的古典诊断方法获得的结果一致，但这里使用的方法能够实现靶生物的快速检测和相对量化，从而协助实施疾病管理策略。

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  • 来源
    《Aerobiologia》 |2019年第2期|201-214|共14页
  • 作者

    Migliorini Duccio; Ghelardini Luisa; Luchi Nicola; Capretti Paolo; Onorari Marzia; Santini Alberto;

  • 作者单位

    Ist Protez Sostenibile Piante CNR Via Madonna del Piano 10 I-50019 Sesto Fiorentino FI Italy;

    Ist Protez Sostenibile Piante CNR Via Madonna del Piano 10 I-50019 Sesto Fiorentino FI Italy|Univ Firenze Dipartimento Sci Prod Agroalimentari & Ambiente D Piazzale Cascine 28 I-50144 Florence Italy;

    Ist Protez Sostenibile Piante CNR Via Madonna del Piano 10 I-50019 Sesto Fiorentino FI Italy;

    Ist Protez Sostenibile Piante CNR Via Madonna del Piano 10 I-50019 Sesto Fiorentino FI Italy|Univ Firenze Dipartimento Sci Prod Agroalimentari & Ambiente D Piazzale Cascine 28 I-50144 Florence Italy;

    Agenzia Reg Protez Ambientale Toscana AFR Aerobiol Pistoia Italy;

    Ist Protez Sostenibile Piante CNR Via Madonna del Piano 10 I-50019 Sesto Fiorentino FI Italy;

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  • 正文语种 eng
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  • 关键词

    Airborne sporangia; TaqMan((R)) MGB probe; Quantitative PCR; Aerial Phytophthora; Disease management;

    机译：空中孢子囊;Taqman（（R））MGB探针;定量PCR;空中植物植物;疾病管理;

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