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Application of for the identification and management of hybrid poplar accessions

机译：在杂种杨树种质鉴定和管理中的应用

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Molecular markers are a powerful tool with many potential applications in agriculture and forestry. In particular, can provide information on the relatedness of various clones or varieties that are difficult to distinguish morphologically, thus helping in the management of plant accessions and in breeding programs. The goal of this study is to genotype 15 clones used in the Prairie Farm Rehabilitation Administration (PFRA) breeding programs. Simple Sequence Repeat (SSR, or microsatellite markers) were selected for genotyping using the polymerase chain reaction (PCR). This type of marker is considered the method of choice due to their abundance, polymorphism and reliability compared to other types of . Sixteen previously isolated and characterized in aspen (Populus tremuloides) and other poplar species (Populus spp.) were initially tested. Nine markers were selected based on the “informativeness” and the quality of the amplification products. The nine markers were combined in groups of three to improve the efficiency of the genotyping technique. Using the nine markers, the average number of alleles per locus was 5.1. The expected and observed heterozygosity ranges were 0.32 to 0.80 and 0.13 to 0.92 respectively. The results also show that it is possible to produce a unique “DNA fingerprint” specific to each of the 15 hybrid poplar clones with the nine . In this study it was possible to show that two clones, P. ‘Melville’ and P. x ‘Walker’ used in Saskatchewan have similar DNA profiles with nine markers and a combined probability of identity of 2.23×10−6 suggesting that these clones are identical. This observation will prevent unnecessary duplication of the two accessions in breeding programs.
机译：分子标记是一种强大的工具，在农业和林业中具有许多潜在应用。特别是，可以提供有关形态上难以区分的各种克隆或变种的相关性的信息，从而有助于植物种质的管理和育种程序。这项研究的目的是对大草原农场恢复管理局（PFRA）育种计划中使用的15个克隆进行基因分型。使用聚合酶链反应（PCR）选择简单重复序列（SSR，或微卫星标记）进行基因分型。与其他类型的相比，此类标记的丰度，多态性和可靠性被认为是选择的方法。最初测试了十六种先前在白杨（Populus tremuloides）中分离并鉴定的特征以及其他杨树物种（Populus spp。）。根据“信息性”和扩增产物的质量选择了九种标记。将这九种标记物分为三个组，以提高基因分型技术的效率。使用这九种标记，每个基因座的平均等位基因数为5.1。预期和观察到的杂合度范围分别为0.32至0.80和0.13至0.92。结果还表明，有可能产生特异的“ DNA指纹”，这些指纹特异于15个杂种杨树克隆中的9个。在这项研究中，有可能表明萨斯喀彻温省使用的两个克隆P.'Melville'和P. x'Walker'具有相似的DNA谱，带有9个标记，并且同一性的合计概率为2.23×10−6 暗示这些克隆是相同的。这种观察将防止在育种程序中不必要地重复这两个种。

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