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首页> 外文期刊>AIDS Research and Human Retroviruses >Short Communication: Limited Induction of IL-10 in PBMCs from HIV-Infected Subjects Treated with HIV-VLPs
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Short Communication: Limited Induction of IL-10 in PBMCs from HIV-Infected Subjects Treated with HIV-VLPs

机译：简短交流：用HIV-VLP治疗的HIV感染受试者的PBMC中IL-10的诱导有限

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摘要

We have recently shown that HIV-1 Pr55gag virus-like particles (HIV-VLPs), produced in a baculovirus expression system and presenting a gp120 molecule from a Ugandan HIV-1 isolate of clade A (HIV-VLPAs), induce maturation and activation of antigen-presenting cells (APCs) in fresh peripheral blood mononuclear cells (PBMCs) from seronegative as well as seropositive, with either low or high viremia, HIV-1 subjects. A Th2 polarization has been observed in both HIV seropositive groups, which is efficiently boosted by HIV-VLP induction and does not switch into a Th1 pattern. Here we show that the production of the known immune-suppressive IL-10 is induced in both HIV-seropositive groups at a significantly lower level by HIV-VLPs compared to LPS. These levels, however, appear to still negatively interfere with the innate as well as adaptive Th1-polarized response observed in HIV-seropositive groups. These results indicate that vaccines and novel adjuvants (i.e., TLR agonists, such as LPS) must be evaluated not only for their immunogenicity but also for their potential immune-suppressive effects. In this perspective, fresh ex vivo PBMCs can be of high value for screening the responses as well as eventual failures of vaccinees enrolled in clinical trials.
机译：我们最近发现，杆状病毒表达系统中产生了HIV-1 Pr55gag病毒样颗粒（HIV-VLP），并呈现出乌干达进化枝A的HIV-1分离株的gp120分子（HIV-VLP A s），可诱导低病毒血症或高病毒血症的HIV-1受试者血清反应阳性和血清反应阳性的新鲜外周血单核细胞（PBMC）中抗原呈递细胞（APC）的成熟和激活。在两个HIV血清阳性组中均观察到Th2极化，HIV-VLP诱导可有效促进Th2极化，并且不会转变为Th1模式。在这里，我们显示，与LPS相比，HIV-VLP在两个HIV血清阳性组中均以较低的水平诱导了已知的免疫抑制性IL-10的产生。然而，这些水平似乎仍会对在HIV血清反应阳性组中观察到的先天性和适应性Th1极化反应产生负面影响。这些结果表明，不仅必须评估疫苗和新型佐剂（即TLR激动剂，例如LPS）的免疫原性，还必须评估其潜在的免疫抑制作用。从这个角度来看，新鲜的离体外周血单个核细胞对于筛选反应以及最终参加临床试验的疫苗的失败可能具有很高的价值。

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  • 来源
    《AIDS Research and Human Retroviruses》 |2009年第8期|819-822|共4页
  • 作者

    L. Buonaguro; M.L. Tornesello; George K. Jewis; F.M. Buonaguro;

  • 作者单位

    Laboratory of Molecular Biology and Viral Oncogenesis and AIDS Reference Center, Istituto Nazionale Tumori “Fond. G. Pascale,” Naples, Italy.;

    Laboratory of Molecular Biology and Viral Oncogenesis and AIDS Reference Center, Istituto Nazionale Tumori “Fond. G. Pascale,” Naples, Italy.;

    Institute of Human Virology, University of Maryland, Baltimore, Maryland.;

    Laboratory of Molecular Biology and Viral Oncogenesis and AIDS Reference Center, Istituto Nazionale Tumori “Fond. G. Pascale,” Naples, Italy.;

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