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首页> 外文期刊>American Fern Journal >Characterization of Four Members of the Alpha-Tubulin Gene Family in Ceratopteris richardii
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Characterization of Four Members of the Alpha-Tubulin Gene Family in Ceratopteris richardii

机译：拟南芥中Alpha-Tubulin基因家族的四个成员的表征

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Four members of the alpha-tubulin gene family were examined in Ceratopteris richardii. Genetic linkage mapping based on a population of nearly 500 Doubled Haploid Lines was able to position three or four members of this gene family on linkage groups 17, 24, and 28, respectively (two of the four observed polymorphic restriction fragments containing alpha-tubulin genes are either identical or map too close to each other on linkage group 17 to be distinguishable in map distance). Non-mapable monomorphic bands observed on probed Southern blots suggest that the alpha-tubulin gene family in this species is large. Four alpha-tubulin genes from C. richardii were sequenced and found to be fairly similar to each other in terms of their amino acid sequences, with their greatest diversity at the carboxy-terminal ends. BLAST comparisons found each of these four amino acid sequences more similar to an alpha-tubulin from a dicot, gymnosperm, or alga species than it was to any other alpha-tubulin sequence presently known from Ceratopteris or from the fern Anemia phyllitidis or the moss Physcomitrella patens. Bayesian phylogenetic analysis of nucleotide sequences placed three of the four Ceratopteris alpha-tubulin gene copies in a clade with copies from Pseudotsuga and Anemia, consistent with a history of two gene duplication events, one following and one preceding the divergence of ferns and seed plants. The fourth copy is robustly separated from the preceding three and placed in a clade of algal alpha-tubulin genes, suggesting its divergence from the ancestor of the other three before the divergence of algae and land plants. As characterized thus far, the alpha-tubulin gene family of C. richardii is relatively large as compared to the six copies known from fully sequenced Arabidopsis thaliana, a condition that may be correlated with the large genome size and diverse life history constraints of this homosporous fern species. These findings suggest several new opportunities for research into the evolution, function, and regulation of the alpha-tubulin gene family in Ceratopteris.
机译：在Ricardopteris richardii中检查了α-微管蛋白基因家族的四个成员。基于近500个双倍单倍体系的种群进行的遗传连锁作图能够将该基因家族的三个或四个成员分别定位在连锁群17、24和28上（观察到的四个包含α-微管蛋白基因的多态性限制性片段中的两个要么是相同的，要么是在链接组17上的地图彼此之间过于接近而无法在地图距离上进行区分）。在探测的Southern印迹上观察到的不可映射的单态性条带表明，该物种中的α-微管蛋白基因家族很大。对来自南美白屈线虫的四个α-微管蛋白基因进行了测序，发现它们的氨基酸序列彼此非常相似，在羧基末端具有最大的多样性。 BLAST比较发现，这四个氨基酸序列中的每个氨基酸序列都与双子叶植物，裸子植物或藻类物种的α-微管蛋白相似，而不是与现今从翅翅目，蕨类贫血或蕨类植物贫血症或苔藓中得到的任何其他α-微管蛋白序列相似。帕滕。核苷酸序列的贝叶斯系统进化分析将四个拟翅目α-微管蛋白基因拷贝中的三个与假拟tsu虫和贫血的拷贝放置在进化枝中，这与两个基因重复事件的历史一致，一个是蕨类植物和种子植物发散的一个，另一个是在此之前。第四个副本与前三个副本牢固地分开，并放置在藻类α-微管蛋白基因的进化枝中，这表明其在藻类和陆地植物分化之前与其他三个祖先的物种分化。到目前为止，与从完全测序的拟南芥已知的六种拷贝相比，RICHARDii的α-微管蛋白基因家族相对较大，这种情况可能与该同源性的大基因组大小和不同的生活史限制有关蕨类。这些发现为研究角翅目中α-微管蛋白基因家族的进化，功能和调控提供了一些新的机会。

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