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首页> 外文期刊>American journal of applied sciences >Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR
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Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR

机译：RAPD-PCR鉴定塔伊夫州桃子品种的遗传多样性

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摘要

Problem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, and fruiting indices were determined and eight, 10-base primers were used to amplify DNA from each cultivar by using Randomly Amplified Polymorphic DNA (RAPD) technology. RAPD profiles (i.e., cultivars) and RAPD amplicons (i.e., markers) were biclustered by using appropriate software and RAPD biclusters were viewed as a heatmap in order to improve the display of RAPD markers. Results: The different RAPD primers produced a total of 114 fragments (amplicons), of flowering which 63 were polymorphic among the studied cultivars. The RAPD fingerprinting results confirmed the data obtained from morphological analyses and allowed for estimating the genetic relatedness among the studied peach cultivars. The data indicated that certain molecular markers might be associated with certain commercial characteristics. Conclusion/Recommendations: Future studies on the association(s) of the selected molecular markers with fruiting characteristics can allow for new gene discovery, molecular breeding, proper biodiversity assessment and better conservation of germplasm resources.
机译：问题陈述：来自沙特阿拉伯王国的桃基因型在生物学和分子水平上没有得到充分表征。这项研究的目的是在生物和分子水平上表征9种在塔伊夫（Ka）种植的桃子的特征。方法：对于研究的9个桃子品种，确定了结实指数，并使用8个10个碱基的引物，通过使用随机扩增多态性DNA（RAPD）技术扩增每个品种的DNA。使用适当的软件将RAPD配置文件（即品种）和RAPD扩增子（即标记）进行二聚化，并将RAPD二聚体作为热图，以改善RAPD标记的显示。结果：不同的RAPD引物共产生114个片段（扩增子），其中有63个为多态性。 RAPD指纹图谱的结果证实了从形态学分析获得的数据，并可以估计所研究桃子品种之间的遗传相关性。数据表明某些分子标记可能与某些商业特性有关。结论/建议：对所选分子标记与结果特征的关联的进一步研究可以发现新基因，分子育种，适当的生物多样性评估和更好地保护种质资源。

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