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首页> 外文期刊>American journal of applied sciences >Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR | Science Publications
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Characterization of the Genetic Diversity of Peach Cultivars in Taif by RAPD-PCR | Science Publications

机译：RAPD-PCR鉴定塔伊夫州桃子品种的遗传多样性科学出版物

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> Problem statement: Peach genotypes from the Kingdom of Saudi Arabia were not adequately characterized at the biological and molecular levels. This study was carried out to characterize nine peach cultivars growing in Taif (KSA) at the biological and molecular levels. Approach: For the nine peach cultivars studied, flowering and fruiting indices were determined and eight, 10-base primers were used to amplify DNA from each cultivar by using Randomly Amplified Polymorphic DNA (RAPD) technology. RAPD profiles (i.e., cultivars) and RAPD amplicons (i.e., markers) were biclustered by using appropriate software and the RAPD biclusters were viewed as a heatmap in order to improve the display of RAPD markers. Results: The different RAPD primers produced a total of 114 fragments (amplicons), of which 63 were polymorphic among the studied cultivars. The RAPD fingerprinting results confirmed the data obtained from the morphological analyses and allowed for estimating the genetic relatedness among the studied peach cultivars. Moreover, the data indicated that certain molecular markers might be associated with certain commercial characteristics. Conclusion/Recommendations: Future studies on the association(s) of the selected molecular markers with fruiting characteristics should allow for new gene discovery, molecular breeding, proper biodiversity assessment and better conservation of germplasm resources.
机译： > 问题陈述：沙特阿拉伯王国的桃基因型在生物学和分子水平上没有得到充分表征。这项研究的目的是从生物学和分子水平上表征9种在塔伊夫（Taaf）（KSA）生长的桃子品种。 方法：对于所研究的9个桃子品种，确定了开花和结果指数，并通过随机扩增多态性DNA（RAPD）技术使用了8个10个碱基的引物从每个品种中扩增DNA。通过使用适当的软件将RAPD配置文件（即品种）和RAPD扩增子（即标记）进行二聚化，并将RAPD二聚体视为热图，以改善RAPD标记的显示。 结果：不同的RAPD引物共产生114个片段（扩增子），其中63个为多态性。 RAPD指纹图谱的结果证实了从形态学分析获得的数据，并可以估计所研究桃子品种之间的遗传相关性。此外，数据表明某些分子标记可能与某些商业特性有关。 结论/建议：有关所选分子标记与结果特征的关联的未来研究应考虑到新基因的发现，分子育种，适当的生物多样性评估和更好地保护种质资源。

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