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首页> 外文期刊>Annals of Biomedical Engineering >Intervertebral Disc Cell Therapy for Regeneration: Mesenchymal Stem Cell Implantation in Rat Intervertebral Discs
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Intervertebral Disc Cell Therapy for Regeneration: Mesenchymal Stem Cell Implantation in Rat Intervertebral Discs

机译:椎间盘细胞再生治疗:大鼠椎间盘间质干细胞植入。

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摘要

This study explores the use of mesenchymal stem cells (MSCs) for intervertebral disc regeneration. We used an in vivo model to investigate the feasibility of exogenous cell delivery, retention, and survival in the pressurized disc space. MSC injection into rat coccygeal discs was performed using 15% hyaluronan gel as a carrier. Injections of gel with or without MSCs were performed. Immediately after injection, fluorescently labeled stem cells were visible on sections of cell-injected discs. Seven and 14 days after injection, stem cells were still present within the disc, but their numbers were significantly decreased. At 28 days, a return to the initial number of injected cells was observed, and viability was 100%. A trend of increased disc height compared to blank gel suggests an increase in matrix synthesis. The results indicate that MSCs can maintain viability and proliferate within the rat intervertebral disc.
机译:这项研究探索了间充质干细胞(MSCs)用于椎间盘再生。我们使用了体内模型来研究外源细胞在加压椎间盘中的传递,保留和存活的可行性。使用15%透明质酸凝胶作为载体,将MSC注射入大鼠尾椎间盘。进行带有或不带有MSC的凝胶注射。注射后立即在细胞注射盘的切片上可见荧光标记的干细胞。注射后第7天和第14天,椎间盘内仍存在干细胞,但其数量明显减少。在28天时,观察到注射细胞的初始数目返回,并且活力为100%。与空白凝胶相比,椎间盘高度增加的趋势表明基质合成增加。结果表明,间充质干细胞可以维持活力并在大鼠椎间盘内增殖。

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