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首页> 外文期刊>Annals of Biomedical Engineering >Dynamic Measurements of Transverse Optical Trapping Force in Biological Applications
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Dynamic Measurements of Transverse Optical Trapping Force in Biological Applications

机译:生物应用中横向光学陷波力的动态测量

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Optical tweezers present a technology for measurements of biological forces in the piconewton range. In such applications, one method of calibrating the transverse optical trapping force involves relating a known external force to the displacement of the trapped object from the trapping center. In this work we used Fourier analysis of the equation of motion to calculate the displacement of the trapped object from the trapping center under an external force induced by viscous drag. Triangular waveforms of different frequencies were used both in theoretical modeling and experiments to induce a force on a trapped object. We investigated the contribution of various factors including frequency of the external force, fluid viscosity, density, and dimensions of the trapped object, stiffness of the optical trap, and frequency response of the instruments used to control the motion of the viscous medium to the accuracy of the calibration. The developed model can be adopted for calibration of the transverse trapping force, analysis of the trapped object motion, and reconstruction of a force profile during measurements of dynamic biological forces.
机译:光学镊子提出了一种在皮微微范围内测量生物力的技术。在这样的应用中,校准横向光学捕获力的一种方法涉及将已知的外力与被捕获物体从捕获中心的位移相关联。在这项工作中,我们使用了运动方程的傅立叶分析来计算在粘性阻力引起的外力作用下被捕物体从捕集中心的位移。在理论建模和实验中均使用了不同频率的三角波形,以在被困物体上产生力。我们研究了各种因素的作用,包括外力的频率,流体粘度,被捕物体的密度和尺寸,光阱的刚度以及用于控制粘性介质运动到精度的仪器的频率响应校准。所开发的模型可用于校准横向诱集力,分析被诱集物体的运动以及在动态生物力测量过程中重建力分布。

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