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首页> 外文期刊>Apoptosis >Enhanced cell survival and diminished apoptotic response to simulated ischemia–reperfusion in H9c2 cells by magnetic field preconditioning
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Enhanced cell survival and diminished apoptotic response to simulated ischemia–reperfusion in H9c2 cells by magnetic field preconditioning

机译：通过磁场预处理提高了H9c2细胞对模拟缺血再灌注的细胞存活率并减少了其凋亡反应

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摘要

The potential for 60 Hz magnetic field (MF) preconditioning to protect heart-derived, H9c2 cultures from damage by simulated ischemia and reperfusion (I–R) was examined. The most effective MF exposure conditions (120 μT, 4–8 h) increased cell survival by 40–50 % over that seen with I–R alone. Potential targets of MF preconditioning were assessed by investigating the apoptosis-related drop in Bcl-2 levels and elevation of the specific activities of caspases 3, 8 and 9 produced by I–R. In response to MF exposure Bcl-2 levels rose 2 to 2.6-fold, and caspase specific activities fell 51–72 % from the values seen after I–R alone. Levels of Hsp’s 25, 32 and 72 were examined in response to the MF, but showed little-to-no elevation beyond that produced by I–R. However, MF preconditioning produced a 77 % decrease in the I–R-induced translocation of phosphorylated Hsp25 (Hsp25-P) from the cytosolic to the nuclear-cytoskeletal cell fraction. This might protect by maintaining active Hsp25-P in the cytosol to function as a chaperone or to bind cytochrome c. Blocking Hsp25 phosphorylation with SB203580, an inhibitor of p38 MAPK, resulted in increases of 64 and 80 % in the respective specific activities of caspases 3 and 9 in cells subjected to I–R, and eliminated the MF-induced reduction in caspase 3 activity.
机译：检查了60 Hz磁场（MF）预处理保护心脏来源的H9c2培养物免受模拟缺血和再灌注（IR）损害的可能性。与仅使用IR相比，最有效的MF暴露条件（120μT，4–8 h）可使细胞存活增加40–50％。通过研究细胞凋亡相关的Bcl-2水平下降以及I–R产生的胱天蛋白酶3、8和9的比活性升高，来评估MF预处理的潜在目标。响应MF暴露，Bcl-2水平上升了2倍至2.6倍，而半胱天冬酶的比活度比仅在I–R之后的数值下降了51–72％。检查了Hsp的25、32和72的水平，以响应MF，但显示出超出I–R的升高几乎没有或没有升高。但是，MF预处理使I-R诱导的磷酸化Hsp25（Hsp25-P）从胞质向核-骨架细胞部分的转运减少了77％。这可能通过在细胞质溶胶中维持活性Hsp25-P来充当伴侣或结合细胞色素c来保护。用p203 MAPK抑制剂SB203580阻断Hsp25磷酸化，可使I-R细胞中胱天蛋白酶3和9的比活性分别增加64％和80％，并消除了MF诱导的caspase 3活性降低。

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