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首页> 外文期刊>Applied Magnetic Resonance >Pulsed EPR Analysis of the Photo-Induced Triplet Radical Pair in the BLUF Protein SyPixD: Determination of the Protein–Protein Distance and Orientation in the Oligomeric Protein
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Pulsed EPR Analysis of the Photo-Induced Triplet Radical Pair in the BLUF Protein SyPixD: Determination of the Protein–Protein Distance and Orientation in the Oligomeric Protein

机译：对BLUF蛋白SyPixD中的光诱导三重态自由基对的脉冲EPR分析：低聚蛋白中蛋白质-蛋白质的距离和方向的确定

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摘要

A photo-induced radical pair of FADH· and Y8· and in BLUF protein SyPixD was studied by pulsed electron paramagnetic resonance (EPR) spectroscopy. Blue light illumination at 150 K for 30 min followed by cooling to 50 K during illumination induced the stable radical pair. The EPR signal has been characterized by a Pake doublet signal with complete S = 1 spin state. The radical pair was utilized as a probe to analyze the oligomer of SyPixD. The relative arrangement of PixD proteins in the complex was investigated by pulsed electron–electron double resonance (PELDOR) with the orientation selection. Based on the decameric structure in the crystal, the possible structure for the PELDOR results was discussed.
机译：通过脉冲电子顺磁共振（EPR）光谱研究了FADH ·和Y8 ·以及BLUF蛋白SyPixD中的光诱导自由基对。在150 K下蓝光照射30分钟，然后在照射期间冷却至50 K诱导稳定的自由基对。 EPR信号的特征是具有完全S = 1自旋状态的Pake doublet信号。自由基对被用作探针来分析SyPixD的低聚物。 PixD蛋白在复合物中的相对排列是通过脉冲电子-电子双共振（PELDOR）和方向选择来研究的。基于晶体的十聚体结构，讨论了可能的PELDOR结果结构。

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