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首页> 外文期刊>Applied Magnetic Resonance >Pulsed EPR Analysis of the Photo-Induced Triplet Radical Pair in the BLUF Protein SyPixD: Determination of the Protein-Protein Distance and Orientation in the Oligomeric Protein
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Pulsed EPR Analysis of the Photo-Induced Triplet Radical Pair in the BLUF Protein SyPixD: Determination of the Protein-Protein Distance and Orientation in the Oligomeric Protein

机译：BLUF蛋白SyPixD中光诱导三重态自由基对的脉冲EPR分析：寡聚蛋白中蛋白质-蛋白质的距离和方向的确定

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摘要

A photo-induced radical pair of FADH· and Y8· and in BLUF protein SyPixD was studied by pulsed electron paramagnetic resonance (EPR) spectroscopy. Blue light illumination at 150 K for 30 min followed by cooling to 50 K during illumination induced the stable radical pair. The EPR signal has been characterized by a Pake doublet signal with complete S = 1 spin state. The radical pair was utilized as a probe to analyze the oligomer of SyPixD. The relative arrangement of PixD proteins in the complex was investigated by pulsed electron-electron double resonance (PELDOR) with the orientation selection. Based on the decameric structure in the crystal, the possible structure for the PELDOR results was discussed.
机译：通过脉冲电子顺磁共振（EPR）光谱研究了FAUF·和Y8·以及BLUF蛋白SyPixD中的光诱导自由基对。在150 K下蓝光照射30分钟，然后在照射期间冷却至50 K诱导稳定的自由基对。 EPR信号的特征是具有完全S = 1自旋状态的Pake doublet信号。自由基对被用作探针来分析SyPixD的低聚物。通过选择方向的脉冲电子双共振（PELDOR）研究了PixD蛋白在复合物中的相对排列。基于晶体的十聚体结构，讨论了可能的PELDOR结果结构。

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