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首页> 外文期刊>Applied Microbiology and Biotechnology >Synergistic antidigestion effect of Lactobacillus rhamnosus and bovine colostrums in simulated gastrointestinal tract (in vitro)
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Synergistic antidigestion effect of Lactobacillus rhamnosus and bovine colostrums in simulated gastrointestinal tract (in vitro)

机译：鼠李糖乳杆菌和牛初乳在模拟胃肠道中的协同抗消化作用（体外）

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Probiotics and bovine colostrums had been proven to be beneficial for human health. Lactobacillus rhamnosus ZDY114 and anti-Helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect in the simulated gastrointestinal tract (in vitro) was investigated. Either L. rhamnosus or purified IgG from immune colostrums was very sensitive in simulated gastric environment and slightly sensitive in simulated intestinal tract. No viable counts were recovered from the solution of dissolved freeze-dried powder (7.14 log10 CFU/ml) of L. rhamnosus when digested at pH 3.0 with pepsin for 30 min. Activity of purified IgGs from immune colostrums could not be detected when digested at pH 3.0 with pepsin for 30 min; 29% titer could be detected when digested at pH 8.0 with trypsin for 5 h. The IgGs in nonpurified immune colostrums presented stronger resistance against gastrointestinal digestion than purified IgGs. Moreover, the combination of L. rhamnosus ZDY114 and immune colostrums strengthened their antidigestion ability. Even under pH 3.0, 4.0 with pepsin, the titer of anti-HP IgG maintained above 123 and 83.3%, respectively. Similarly, that titer was above 93.3% when digested at pH 8.0 with trypsin for 4.5 h. In conclusion, L. rhamnosus and anti-Helicobacter pylori bovine colostrums had synergistic antidigestion effect in simulated gastrointestinal tract (in vitro).
机译：益生菌和牛初乳已被证明对人体健康有益。将鼠李糖乳杆菌ZDY114和抗幽门螺杆菌牛初乳用于制备微生态添加剂，并研究了它们在模拟胃肠道中的协同抗消化作用（体外）。鼠李糖乳杆菌或免疫初乳中纯化的IgG在模拟胃环境中非常敏感，而在模拟肠道中则稍敏感。当用胃蛋白酶在pH 3.0下消化30分钟时，鼠李糖乳杆菌的冷冻干燥粉末溶液（7.14 log10 CFU / ml）中没有发现活菌。用胃蛋白酶在pH 3.0下消化30分钟，无法检测到来自免疫初乳的纯化IgG的活性。用胰蛋白酶在pH 8.0消化5小时，可检测到29％的滴度。与纯化的IgG相比，未纯化的免疫初乳中的IgG对胃肠消化的抵抗力更强。此外，鼠李糖乳杆菌ZDY114和免疫初乳的结合增强了它们的抗消化能力。即使在胃蛋白酶的pH 3.0、4.0下，抗HP IgG的滴度也分别保持在123和83.3％以上。同样，当用胰蛋白酶在pH 8.0消化4.5 h时，该效价高于93.3％。总之，鼠李糖乳杆菌和抗幽门螺杆菌牛初乳在模拟胃肠道中具有协同的抗消化作用（体外）。

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