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首页> 外文期刊>Applied Microbiology and Biotechnology >Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes
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Improving lysine production by Corynebacterium glutamicum through DNA microarray-based identification of novel target genes

机译：通过基于DNA芯片的新型靶基因鉴定，提高谷氨酸棒杆菌的赖氨酸生产

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摘要

For the biotechnological production of l-lysine, mainly strains of Corynebacterium glutamicum are used, which have been obtained by classical mutagenesis and screening or selection or by metabolic engineering. Gene targets for the amplification and deregulation of the lysine biosynthesis pathway, for the improvement of carbon precursor supply and of nicotinamide adenine dinucleotide phosphate (reduced form) (NADPH) regeneration, are known. To identify novel target genes to improve lysine production, the transcriptomes of the classically obtained lysine producing strain MH20-22B and several other C. glutamicum strains were compared. As lysine production by the classically obtained strain, which possesses feedback-resistant aspartokinase and is leucine auxotrophic, exceeds that of a genetically defined leucine auxotrophic wild-type derivative possessing feedback-resistant aspartokinase, additional traits beneficial for lysine production are present. NCgl0855, putatively encoding a methyltransferase, and the amtA-ocd-soxA operon, encoding an ammonium uptake system, a putative ornithine cyclodeaminase and an uncharacterized enzyme, were among the genes showing increased expression in the classically obtained strain irrespective of the presence of feedback-resistant aspartokinase. Lysine production could be improved by about 40% through overexpression of NCgl0855 or the amtA-ocd-soxA operon. Thus, novel target genes for the improvement of lysine production could be identified in a discovery-driven approach based on global gene expression analysis.
机译：对于l-赖氨酸的生物技术生产，主要使用谷氨酸棒杆菌菌株，其是通过经典诱变和筛选或选择或通过代谢工程获得的。已知赖氨酸生物合成途径的扩增和去调控，用于改善碳前体供应和烟酰胺腺嘌呤二核苷酸磷酸（还原形式）（NADPH）再生的基因靶标。为了鉴定新的靶基因以改善赖氨酸的产生，比较了经典获得的产生赖氨酸的菌株MH20-22B和其他几个谷氨酸棒杆菌菌株的转录组。由于通过经典获得的具有反馈抗性天冬氨酸激酶并且是亮氨酸营养缺陷型的菌株的赖氨酸生产超过了具有反馈抗性天冬氨酸激酶的遗传定义的亮氨酸营养缺陷型野生型衍生物的赖氨酸生产，因此存在有利于赖氨酸生产的其他性状。假定存在编码甲基转移酶的NCgl0855和编码铵吸收系统，假定鸟氨酸环脱氨酶和未表征的酶的amtA-ocd-soxA操纵子，都是在经典获得的菌株中表达增加的基因，无论是否存在反馈-抗天冬氨酸激酶。通过NCgl0855或amtA-ocd-soxA操纵子的过表达，可将赖氨酸的产量提高约40％。因此，可以在基于全局基因表达分析的发现驱动方法中确定用于改善赖氨酸生产的新型靶基因。

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