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首页> 外文期刊>Applied Microbiology and Biotechnology >Horizontal transfer of genetic determinants for degradation of phenol between the bacteria living in plant and its rhizosphere
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Horizontal transfer of genetic determinants for degradation of phenol between the bacteria living in plant and its rhizosphere

机译：植物和根际中细菌之间苯酚降解的遗传决定因子水平转移

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摘要

Phenol and other monocyclic aromatic compounds (MACs) are highly water-soluble and volatile pollutants that plants are unable to completely degrade. Endophytic bacteria with MAC-degrading ability will facilitate phytoremediation, beneficial to plant survival in contaminated soil. Endophytic bacteria, strains FX1–FX3, and rhizosphere bacteria, strains FX0, FX4, and FX5, were isolated from the root tissue of a corn plant (Zea mays) and the corn rhizosphere near a chemical plant, respectively. The strains FX1–FX5 were able to grow on phenol and reduce phenol concentration, but the strain FX0 was unable to. The strains FX1, FX3, and FX4 were classified as Pseudomonas fluorescens and FX0, FX2, and FX5 as Burkholderia cepacia. The plasmids isolated from the strains FX1–FX5 were found to possess similar traits and to be loaded with a gene encoding the catechol 2, 3-dioxygenase (C23O), a key enzyme in the phenol degradation pathway. Alignment and phylogenetic analysis inferred that in situ horizontal transfer of the C23O gene might have occurred. The horizontal transfer of the C23O gene between endophytic and rhizosphere bacteria was proved by using conjugal matings experiment, in which the transconjugants were found to acquire the plasmid with the C23O gene, able to grow on phenol and degrade phenol.
机译：苯酚和其他单环芳香族化合物（MACs）是高度水溶性和挥发性的污染物，植物无法完全降解。具有MAC降解能力的内生细菌将促进植物修复，有利于植物在污染土壤中的存活。分别从玉米植物（Zea mays）的根组织和化工厂附近的玉米根际中分离出内生细菌FX1-FX3菌株和根际细菌FX0，FX4和FX5菌株。菌株FX1-FX5能够在苯酚上生长并降低苯酚浓度，但菌株FX0无法。菌株FX1，FX3和FX4归类为荧光假单胞菌，而FX0，FX2和FX5归类为洋葱伯克霍尔德菌。发现从菌株FX1-FX5分离的质粒具有相似的性状，并装有编码邻苯二酚2、3-二加氧酶（C23O）的基因，该基因是苯酚降解途径中的关键酶。比对和系统发育分析推断，可能已经发生了C23O基因的原位水平转移。通过共轭交配实验证明了C23O基因在内生细菌和根际细菌之间的水平转移，在该实验中，发现转导结合物获得了具有C23O基因的质粒，该质粒能够在苯酚上生长并降解苯酚。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2007年第3期|733-739|共7页
  • 作者

    Yujing Wang; Ming Xiao; Xiaolu Geng; Jiaying Liu; Jun Chen;

  • 作者单位

    College of Life and Environment Sciences Shanghai Normal University Shanghai 200234 People’s Republic of China;

    College of Life and Environment Sciences Shanghai Normal University Shanghai 200234 People’s Republic of China;

    College of Life and Environment Sciences Shanghai Normal University Shanghai 200234 People’s Republic of China;

    College of Life and Environment Sciences Shanghai Normal University Shanghai 200234 People’s Republic of China;

    College of Life and Environment Sciences Shanghai Normal University Shanghai 200234 People’s Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Pseudomonas fluorescens; Bioremediation; Endophytic bacteria; Rhizosphere bacteria;

    机译：荧光假单胞菌;生物修复;内生细菌;根际细菌;

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