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首页> 外文期刊>Applied Microbiology and Biotechnology >Expression of genes of lipid synthesis and altered lipid composition modulates L-glutamate efflux of Corynebacterium glutamicum
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Expression of genes of lipid synthesis and altered lipid composition modulates L-glutamate efflux of Corynebacterium glutamicum

机译：脂质合成基因的表达和脂质组成的改变调节谷氨酸棒杆菌的L-谷氨酸外排

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L-Glutamate is made with Corynebacterium glutamicum on a scale of more than 106 tons/year. Nevertheless, formation of this amino acid is enigmatic and there is very limited molecular information available to unravel the apparently complex conditions leading to L-glutamate efflux. Here, we report the isolation and overexpression of the genes involved in lipid synthesis: acp, fadD15, cma, cls, pgsA2, cdsA, gpsA, and plsC, and the inactivation of cma and cls. In addition, the consequences for phospholipid content, temperature sensitivity, as well as detergent-independent and detergent-dependent L-glutamate efflux were quantified. An in part strong alteration of the phospholipid composition was achieved; for instance, overexpression of fadD15 encoding an acyl-CoA ligase resulted in an increase of phosphatidyl inositol from 12.6 to 30.2%. All strains, except that overexpressing acp (acyl carrier protein), exhibited increased temperature sensitivity, with the strongest sensitivity present upon cls (cardiolipin synthetase) inactivation. As a consequence of the genetically modified lipid synthesis, L-glutamate efflux changed quite dramatically; for instance, overexpression of plsC (acylglycerolacyl transferase) resulted in a detergent-triggered increase of L-glutamate accumulation from 92 mM to 108 mM, whereas acp overexpression reduced the accumulation to 24 mM. With some of the overexpressed genes, substantial L-glutamate excretion even without detergent addition was obtained when the fermentation temperature was elevated. These data show that the chemical and physical properties of the cytoplasmic membrane are altered and suggest that this is a necessary precondition to achieve L-glutamate efflux.
机译：L-谷氨酸盐是由谷氨酸棒杆菌制得的，规模超过106吨/年。然而，这种氨基酸的形成是令人费解的，并且仅有非常有限的分子信息可用于揭示导致L-谷氨酸外排的表面复杂条件。在这里，我们报道了参与脂质合成的基因的分离和过表达：acp，fadD15，cma，cls，pgsA2，cdsA，gpsA和plsC，以及cma和cls的失活。此外，对磷脂含量，温度敏感性以及不依赖洗涤剂的和依赖洗涤剂的L-谷氨酸外排的后果进行了定量。实现了磷脂组成的部分强烈改变；例如，编码酰基辅酶A连接酶的fadD15的过表达导致磷脂酰肌醇从12.6％增加到30.2％。除过表达acp（酰基载体蛋白）外，所有菌株均表现出更高的温度敏感性，而在cls（心磷脂合成酶）失活时，其敏感性最高。基因修饰的脂质合成的结果是，L-谷氨酸的外排变化非常明显。例如，plsC（酰基甘油糖基酰基转移酶）的过表达导致去污剂触发的L-谷氨酸的积累从92 mM增加到108 mM，而acp过表达将积累减少到24 mM。使用某些过表达的基因，当发酵温度升高时，即使不添加去污剂也能获得大量的L-谷氨酸排泄。这些数据表明细胞质膜的化学和物理性质被改变，并表明这是实现L-谷氨酸外排的必要前提。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2002年第1期|89-96|共8页
  • 作者

    K. Nampoothiri; C. Hoischen; B. Bathe; B. Möckel; W. Pfefferle; K. Krumbach; H. Sahm; L. Eggeling;

  • 作者单位

    Institut für Biotechnologie Forschungszentrum Jülich GmbH 52425 Jülich Germany;

    Department of Molecular Biology Institute of Molecular Biotechnology Beutenbergstrasse 11 07745 Jena Germany;

    Degussa AG Research and Development FA-FE-B 33790 Halle Germany;

    Degussa AG Research and Development FA-FE-B 33790 Halle Germany;

    Degussa AG Research and Development FA-FE-B 33790 Halle Germany;

    Institut für Biotechnologie Forschungszentrum Jülich GmbH 52425 Jülich Germany;

    Institut für Biotechnologie Forschungszentrum Jülich GmbH 52425 Jülich Germany;

    Institut für Biotechnologie Forschungszentrum Jülich GmbH 52425 Jülich Germany;

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