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首页> 外文期刊>Applied Microbiology and Biotechnology >Evaluation of an endo-β-mannanase produced by Streptomyces ipomoea CECT 3341 for the biobleaching of pine kraft pulps
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Evaluation of an endo-β-mannanase produced by Streptomyces ipomoea CECT 3341 for the biobleaching of pine kraft pulps

机译：评估由链霉菌CECT 3341产生的内切β-甘露聚糖酶对松树牛皮纸浆的生物漂白

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摘要

An endo-β-mannanase (EC 3.2.1.78) from Streptomyces ipomoea CECT 3341 was purified and applied to the biobleaching of pine kraft pulps. The maximum level of endo-β-mannanase activity (0.6 units ml–1) was achieved after 4 days of growth in a medium containing locust bean gum and yeast extract. Zymograms revealed mannanase bands (Man) with high and low electrophoretic mobility on the second and seventh days of incubation (Man1, Man3) and three bands of high, medium and low mobility from the third to sixth days of growth (Man1, Man2, Man3). Although these exhibited different molecular masses, their amino-terminal sequences were identical. The action of proteases detected in the culture supernatant could be responsible for such events, suggesting that only one endo-β-mannanase is produced by S. ipomoea. The purified Man3 exhibited a molecular mass of 40 kDa, an isoelectric point of 4.0 and an optimal temperature and pH reaction of 55 °C and 7.5, respectively. It was strongly inhibited by Ag+, Hg2+, Al3+ and Fe3+, and was strongly activated by Mn2+. The ability of the purified endo-β-mannanase to improve the bleachability of pine kraft pulp, when applied with alkaline extraction, was demonstrated by an increase in the pulp brightness (1.7%, using the International Standards Organisation's test) and an absence of variations in the viscosity values. A relationship between the increase in pulp brightness and the presence of manganese in the pulps could be established.
机译：纯化了来自链霉菌CECT 3341的内切-β-甘露聚糖酶（EC 3.2.1.78），并将其用于松树牛皮纸浆的生物漂白。在含有刺槐豆胶和酵母提取物的培养基中生长4天后，β-甘露聚糖酶的最大活性达到0.6个单位ml–1 。字形图显示了在培养的第二天和第七天（Man1，Man3）具有高和低电泳迁移率的甘露聚糖酶谱带（Man），以及在生长的第三天到第六天（Man1，Man2，Man3）具有高，​​中，低迁移率的三个谱带）。尽管它们表现出不同的分子量，但是它们的氨基末端序列是相同的。在培养上清液中检测到的蛋白酶的作用可能是造成这种事件的原因，这表明伊豆链球菌仅产生一种内切-β-甘露聚糖酶。纯化的Man3的分子量为40 kDa，等电点为4.0，最佳温度和pH反应分别为55°C和7.5。它被Ag + ，Hg2 + ，Al3 + 和Fe3 + 强烈抑制，并被Mn2 + 强烈激活。纸浆白度的增加（使用国际标准组织的测试为1.7％），证明了纯化的内切β-甘露聚糖酶改善松树牛皮纸浆的可漂白性的能力（通过国际标准组织的测试）粘度值。可以确定纸浆白度的增加与纸浆中锰的存在之间的关系。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2002年第1期|67-72|共6页
  • 作者

    M. Montiel; M. Hernández; J. Rodríguez; M. Arias;

  • 作者单位

    Departamento de Microbiología y Parasitología Universidad de Alcalá Ctra. Madrid–Barcelona Km 33600 28871 Alcalá de Henares Madrid Spain;

    Departamento de Microbiología y Parasitología Universidad de Alcalá Ctra. Madrid–Barcelona Km 33600 28871 Alcalá de Henares Madrid Spain;

    Departamento de Microbiología y Parasitología Universidad de Alcalá Ctra. Madrid–Barcelona Km 33600 28871 Alcalá de Henares Madrid Spain;

    Departamento de Microbiología y Parasitología Universidad de Alcalá Ctra. Madrid–Barcelona Km 33600 28871 Alcalá de Henares Madrid Spain;

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