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首页> 外文期刊>Applied Microbiology and Biotechnology >Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon TnMERI1
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Simultaneous detection and removal of organomercurial compounds by using the genetic expression system of an organomercury lyase from the transposon TnMERI1

机译：通过转座子TnMERI1的有机汞裂解酶的基因表达系统，同时检测和去除有机汞化合物

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摘要

Using a newly identified organomercury lyase gene (merB3) expression system from TnMERI1, the mercury resistance transposon first found in Gram-positive bacteria, a dual-purpose system to detect and remove organomercurial contamination was developed. A plasmid was constructed by fusing the promoterless luxAB genes as bioluminescence reporter genes downstream of the merB3 gene and its operator/promoter region. Another plasmid, encoding mer operon genes from merR1 to merA, was also constructed to generate an expression regulatory protein, MerR1, and a mercury reductase enzyme, MerA. These two plasmids were transformed into Escherichia coli cells to produce a biological system that can detect and remove environmental organomercury contamination. Organomercurial compounds, such as neurotoxic methylmercury at nanomolar levels, were detected using the biomonitoring system within a few minutes and were removed during the next few hours.
机译：使用新发现的来自TnMERI1的有机汞裂解酶基因（merB3）表达系统，该抗汞转座子首先在革兰氏阳性细菌中发现，开发了一种用于检测和去除有机汞污染的双重用途系统。通过融合无启动子luxAB基因作为merB3基因及其操纵子/启动子区域下游的生物发光报告基因来构建质粒。还构建了另一个编码从merR1到merA的mer操纵子基因的质粒，以产生表达调节蛋白MerR1和汞还原酶MerA。将这两个质粒转化到大肠杆菌细胞中，以产生可以检测和去除环境有机汞污染的生物系统。使用生物监测系统在几分钟内检测到了有机汞化合物，例如纳摩尔水平的神经毒性甲基汞，并在接下来的几个小时内将其去除。

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  • 来源
    《Applied Microbiology and Biotechnology》 |2002年第1期|86-90|共5页
  • 作者

    M. Narita; T. Yamagata; H. Ishii; C.-C. Huang; G. Endo;

  • 作者单位

    Laboratory of Environmental Biotechnology Tohoku Gakuin University 1–13–1 Chuo Tagajo Miyagi 985–8537 Japan;

    Laboratory of Environmental Biotechnology Tohoku Gakuin University 1–13–1 Chuo Tagajo Miyagi 985–8537 Japan;

    Laboratory of Environmental Biotechnology Tohoku Gakuin University 1–13–1 Chuo Tagajo Miyagi 985–8537 Japan;

    Department of Botany National Chung-Hsing University Taichung 40227 Taiwan Republic of China;

    Laboratory of Environmental Biotechnology Tohoku Gakuin University 1–13–1 Chuo Tagajo Miyagi 985–8537 Japan;

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